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Expression of m-RNA of myoD in free muscle graft

山口医学 Volume 52 Issue 6 Page 229-235
published_at 2003-12-31
B030052000603.pdf
[fulltext] 867 KB
Title
機能的筋肉移植術におけるMyoDの発現
Expression of m-RNA of myoD in free muscle graft
Creators Sakamoto Sohtetsu
Source Identifiers
Creator Keywords
筋肉移植 再神経支配 筋再生 MyoD 衛星細胞
We hypothesize that recovery process after free muscle graft activates regeneration of satellite cells in the grafted muscle. The present study was undertaken to prove the hypothesis using MyoD, one of the myogenic regulatory factors (MRFs), which are known as regulators in the process of muscle regeneration after muscle injuries. Models for muscle graft were created using gastrocunemius muscles of Wister rats. They were divided into three groups. The first group was the reinnervation model in which the tibial nerve received neurorraphy. The second group was the denervation model which received the nerve cut only. The third group was the ischemia model. In all groups, one-hour ischemia was given to the muscles. In these three groups, an expression of mRNA of MyoD was examined by RT-PCR. Electromyography confirmed the reinnervation at 4 weeks after the operation in the reinnervation group. The m-RNA expression of MyoD markedly increased at the time of the reinnervation. On the other hand, the amount of the expression in the denervation group was less than half of the first group at 3 weeks after the operation. Marked expression of MyoD mRNA which correlated with the process of reinnervation in the muscle graft strongly suggested that activation of satellite cells were involved in these processes.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 2003-12-31
Rights
本文データは山口大学医学会の許諾に基づきCiNiiから複製したものである()
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1731
[NCID]AN00243156
[isVersionOf] [NAID]http://ci.nii.ac.jp/naid/110002773857/
Schools 医学部