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The Evoked Potential in the Slice of Guinea Pig Hippocampus, and the Effects of Zinc on It

The bulletin of the Yamaguchi Medical School Volume 17 Issue 1-2 Page 33-43
published_at 1970-06
A020017000103.pdf
[fulltext] 2.2 MB
Title
The Evoked Potential in the Slice of Guinea Pig Hippocampus, and the Effects of Zinc on It
Creators Tsue Mitsumaro
Source Identifiers
1) A thin slice was prepared from the hippocampus og guinea pig. It was activated by the direct electrical stimulus and the potential was recorded at the dissected surface nearby the stimulating point. 2) The recorded potential, called as evoked one in the present paper, was biphasic : i. e. a slow negative wave of 200~400 msec in duration was followed by a positive one of about the same duration and small amplitude. The potential reversed in polarity, when the slice was inverted and it was recorded from the surface of pia mater. 3) Acetylcholine (1×10-6 M), in the presence of physostigmine, depressed the evoked potential in amplitude, but the potential recovered in 10 minutes when the slice was washed with the Krebs-Ringer's solution discontaining the drug (normal solution). 4) When chloride ion in the incubating medium was completely replaced by propionate ion, the same stimulus provoked a train of discharges, which could not be observed after washing with the normal solution. 5) In the presence of zinc chloride, the decreaseof ampitude and the prolongation af the duration of the potential, especially the slowing of faling phase, were observed. The threshold concentration was 5×10-6 molar. The changes of the potential did not recover after washing. 6) A block of impilse-transmission at the presynaptic terminal, a change in excitability of postsynaptic membrane and/or of neuron-soma and an alteration of inhibitory synaptic action of afferent impulse were discussed as the possible affective mechanisms of zinc.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1970-06
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部