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DISTRIBUTION OF ALKALINE PHOSPHATASE IN THE PROTEINS OF BOVINE LYMPH-NODES

The bulletin of the Yamaguchi Medical School Volume 5 Issue 3 Page 93-102
published_at 1957-12-31
A020005000301.pdf
[fulltext] 695 KB
Title
DISTRIBUTION OF ALKALINE PHOSPHATASE IN THE PROTEINS OF BOVINE LYMPH-NODES
Creators Takahashi Motoyuki
Source Identifiers
Homogenate of bovine lymph-nodes was extracted with the same volime of physiological saline solution and acidified to pH 4.5. The extract obtained was fractionated with ethanol. The ethanol fraction obtained by the addition of one third to one half of its volume of alcohol was extracted with water and fractionated by electrophorsis convection, using citrate buffer of pH 6.0 and ionic strenght 0.05. Thephosphatase-activity per unit of nitrogen was higher for the fraction from the top reservoir than for the one from the bottom reservoir. All the fractions obtained at different stages in the procedure of purification were analyzed by electrophoresis. It was found that the alkaline phosphatase of bovine lymph-node was contained in the protein fraction of lower mobility.
Subjects
獣医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1957-12-31
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部