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MIG and I309 accelerates proliferation of human bone marrow-derived mesenchymal stem cells

The bulletin of the Yamaguchi Medical School Volume 67 Issue 1-2 Page 1-10
published_at 2020
A050067000101.pdf
[fulltext] 1.56 MB
Title
MIG and I309 accelerates proliferation of human bone marrow-derived mesenchymal stem cells
Creators Aibe Yuki
Creators Takami Taro
Creators Fujisawa Koichi
Creators Matsumoto Toshihiko
Creators Sakaida Isao
Source Identifiers
Creator Keywords
mesenchymal stem cell MIG I309 cell proliferation regenerative medicine
Background: Previous studies have shown that mesenchymal stem cells (MSCs) are an important source of somatic stem cells for regenerative medicine. Therefore, the establishment of efficient MSC culture methods to increase their therapeutic value is essential. Here, we focused on cytokines as accelerators of cell proliferation. Methods: We evaluated the effect of the supernatant—obtained from a co-culture of bone marrow-derived MSCs (BMSCs) and myeloid cells—on the proliferation of BMSCs. We then screened for cytokines present in high concentrations in the medium. Next, we added the identified cytokines to the culture medium in various combinations, and cultured BMSCs in this medium to evaluate the effects of cytokines on their proliferation and differentiation potential. Results: The medium obtained from the co-culture of BMSCs with myeloid cells stimulated BMSC proliferation. Nineteen cytokines were present in high concentrations in the medium. Out of the 19 cytokines, MIG and I309 stimulated BMSC proliferation. Furthermore, the culture medium supplemented with MIG and I309 maintained the differentiation potential of cultured BMSCs. Conclusions: MIG and I309 stimulate BMSC proliferation while maintaining their differentiation potential. These results may contribute to the establishment of more efficient MSC culture methods than those currently being used.
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University School of Medicine
Date Issued 2020
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 大学院医学系研究科(医学)