Effects of Prostaglandin E_1 on Energy Metabolism and Hemodynamics of the Liver in Acute Bleeding in Obstructive Jaundiced Rats

The protective effect of prostaglandin Ej against liver tissue injury was examined in acute bleeding(1% of body weight)models of 24 hour obstructive jaundiced rats. To assess the liver metabolic changes after blood letting, we used noninvasive in vivo <31>^P-MRS. The changes in inorganic phosphate(Pi) and β-ATP peaks (expressed as the height of signals) were monitored and analyzed. We also observed the changes in the chemical shifts of Pi signals relative to α-ATP signals. Simultaneously, to assess the hepatic hemodynamics. the hepatic tissue blood hemoglobin concentration(IHb) , oxygen saturation of hemoglobin(ISO_2) , and consumption of oxygen(IC-O_2) in situ were estimated by reflectance spectrophotometry. The ratio of β-ATP/Pi in the PGE_1 treated group was significantly higher than that in the non-treated group and chemical shifts in the PGE_1 group remained relatively low after blood letting. The ISO_2, IC-O_2 values in the PGE_1 group were significantly higher than those in the non-treated group at 60 min after blood letting. These values were similar to the original values.The results of experimental studies suggest that a small amount of bleeding in obstructive jaundice is dangerous and PGE_1 may protect hepatic metabolic disorder and prevent acidotic changes of liver cells from bleeding during surgery in patients with obstructive jaundice. The effects of PGE_1 were considered to be associated with the maintenance of ISO_2, IHb and IC-O_2.

閉塞性黄疸

Prostaglandin E_1

エネルギー代謝

<31>^P-MRS法

臓器反射スペクトル法