The Influence of Chemotherapy on Proliferating Cell Nuclear Antigen of Glioblastoma Cell Line

In this study we analized cell kinetics of glioblastoma cell line (T98G) under growth suppresive state by use of PCNA-DNA and BrdU-DNA simultaneous double stain method (flow cytometry). The growth suppresive state was induced by two kinds of anticancer agents of MCNU and etoposide. In spite of decrease of BrdU antigen by both anticancer agents, PCNA antigen and PCNA positive ratio increased compared with that of control in the early stage of chemotherapy. Especially PCNA antigen was increased more than two times of control by the proliferation suppresive concentration of etoposide that acted after consumption of PCNA in the series of DNA synthesis. We considered that was caused by change of cell cycle, negative feedback against growth suppresive state and suppression of comsumption of PCNA. It can be summarized that PCNA antigen and PCNA positive ratio under growth suppresive state by anticancer agent don't reflect truly tumor proliferation in the early stage of chemotherapy, and we can't judge the effect of anticancer agent by analysis of cell kinetics used PCNA. In the future, compared with control growth suppresive state would be caught in early stage of chemotherapy by increase of PCNA antigen.

glioblastoma cell line

chemotherapy

proliferating cell nuclear antigen