Studies on the Specimen Preparation Method for Scanning Electron Microscopy PartV. Special Treatment for Specimen Peparation. 1. Preparation on the Specimen for Observing of the Same and Fixed Opical Field by Light Microscope and Scannig Electron …

In order to observe the same and fixed opical field in the specimen by both light microscope (LM) and scanning electron microscope (SEM) , some pretreatments were contrived for specimen preparation. That is the marking technique on the glass slide piece by using object marker was adopted. Moreover a system of technical procedures, such as light microscopy and photography by using the dry or water immersion system of object lens, searhing and triming of the same and fixed opical field on the SEM-screen by collating of both images with printed LM-graph obtaining of observations after printing of both images, was established. The usefullness of this system was shown to be practical by using some samples. Therefore, the simplicity and certainty of this system is considered to be worthy of utility.