Analysis of cerebrospinal fluid proteins reveals association of calbindin 1 concentrations with neurological outcomes in patients resuscitated from out-of-hospital cardiac arrest : a proteomics-based pilot study

The purpose of this study is to establish an analytical platform for identifying a major subset of cerebrospinal fluid (CSF) proteins in patients resuscitated from out-of-hospital cardiac arrest and evaluating their associations with neurological outcomes. Thirteen patients, who were resuscitated from cardiac arrest and survived for more than 48 hours, were enrolled and their CSF was obtained. Patients were classified into favorable (group F) and unfavorable (group U) outcomes based on the Glasgow Outcome Scale at 6 months after the return of spontaneous circulation.Control CSF was also obtained from 8 subjects with no obvious neurological disorders (group N). Protein identification was performed on one individual from each group by liquid chromatography-tandem mass spectrometry and verified on all 21 individuals by western blotting. In total, 107 proteins were identified in CSF. Of those, 40 proteins were identified only in the group U patients, including brain-specific proteins,such as enolase 2 and calbindin 1. The levels of calbindin 1 in individual CSF were significantly higher in group U than in group N or in group F, and associated with the Glasgow Outcome Scale scores. A combination of liquid chromatography-tandem mass spectrometry and western blotting is useful to analyze CSF from critical care unit patients.

cerebrospinal fluid

proteomic analysis

cardiopulmonary arrest

calbindin 1

biomarker