The bulletin of the Yamaguchi Medical School
Continued by：Medical Science & Innovation
EISSN：2758-5441
Back to Top
Permalink : https://petit.lib.yamaguchi-u.ac.jp/350
The bulletin of the Yamaguchi Medical School Volume 48 Issue 3-4
published_at 2001-12
A role of membrane type-1 matrix metalloproteinase in the regulation of vascular smooth muscle cell proliferation in vitro
A role of membrane type-1 matrix metalloproteinase in the regulation of vascular smooth muscle cell proliferation in vitro
Hua Zyang
About This Article
Descriptions
The purpose of this study was to assay changes in membrance type-1 matrix metalloproteinase (MT1-MMP) in vivo after vascular injury and to determine the influence of antisense MT1-MMP oligonucleotide on vascular smooth muscle cell proliferation and migration in vitro. mRNA expression of MT1-MMP was investigated in balloon-injured rat common carotid artery. The effect of antisense MT1-MMP oligonucleotide on activated by zymography. The influence of MT1-MMP antisense oligonucleotide on the proliferation and migration of cultured SMCs was measured by3-(4, 5-dimethylthiazol-2yl)2, 5-diphenyl tetrasodium bromine assay and a 24-well transwell cell culture chamber method. The expression of MT1-MMP mRNA was increased in injured arteries, reaching a peak increace at 4 days after inhibited slightly by antisense MT1-MMP oligonucletide. Antisense MT1-MMP oligonucleotide significantly inhibited SMC proliferation (p＜0.05) but had little effect on the migration of cultured SMCs. Expression of MT1-MMP mRNA increases following arterial injury. Antisense MT1-MMP oligonucleotide regulates SMC proliferation and may therefore constitune a featment for inhibition of intimal hyperplasia.
Creator Keywords
smooth muscle cell prolifeeration
migration
membrane type-1 matrix metalloproteinase
matrix metalloproteinase-2
antisense
Other Article