Inducible Particles of Bacillus aneurinolyties．

To detect prophages and bacteriocins, twenty strains of Bacillus aneurinolyticus were treated with mitomycin C. The result ed lysates were subjected to electron microscopy, and also examined for killing and plaque-forming activities. Seventeen strains showed killing activity on two or more strains of Bacillus aneurinolyticus. Killing agents were centrifuged in linear 5 to 20% sucrose gradient, and studied with electron microscopy which revealed the presence of particles. They looked morphologically like phage tail of 190 nm long with fiber (KA10,KA17) or without fiber (KA1,KA6), T even phage-like particle with a head of 50 nm in diameter and a tail of 140 nm long (KA23), or T7 phage-like partocle with a head of 70 nm in diameter and a tail of 20 nm long (KAS232). The killing agent of KAS232 showed plaque-forming activity on several strains different from killing sensititive strains of Bacillus aneurinolyticus.

Bacillus bacteriocin

bacteriophage

mitomycin C

electron microscopy.