Monoclonal Antibodies against Fetal Rat Liver Cells

The clones that produce the monoclanal antibodies to fetal rat liver cells were established by fusing spleen cells from immunized BALB/c mouse with NS-1 myeloma cells. The characteristics of the monoclonal antibodies (UB-11 and UB-12) to fetal rat liver cells were analyzed by cellular radioimmunoassay, flow cytofluorography and indirect immunoperoxidase procedures: UB-11 and UB-12 recognised preferentially the cell membrane of hemopoietic cells in the fetal liver. UB-11 might be defferent from UB-12, as UB-11 cross-reacted with red blood cells, while UB-12 did not. The usefulness of these two monoclonal antibodies to analyze the differentiation of the rat hemopoietic cells to lymphocytes, granulocytes or red blood cells was discussed.

monoclonal antibodies

fetal liver

celluar radioimmunoassay

flow cytofluorography

indirect immunoperoxidase procedure