Cell Cycle Dependent Con－A binding－Flow Cytometric Analysis－

Changes in lectin binding activity occuring the cell cycle were determined flow cytometrically in exponentaiiy growing YU-101 cells by a technique of gated analysis based on cellular DNA content after double staining of the cells with fluorescein isothiocyanate (FITC) and propidium iodide (PI). Fluorescence intensity from FITC conjugated concanavalin A (Con A) increased during the cell cycle and reached maximum in G2+M phase. Binding of the lectins was reduced by half in G1 phase after cell division. Binding activity of Con A and in YU-101 cells was linked closely with cell cycle. It is surrested that the nuclear changes are associated with alterations of the cellular membrane and that molecular communications between the cell membrane and the cell nucleus control periodic cell cycle. The methodology developed in these studies gives reliable data since a procedure for cell synchronization which brings out some effects on the normal function of the cell cycle is unnecessary.

Con A

Flow cytometry

Cell Cycle

DNA