Distributions of Catecholamines in the Heart of Mammalian Laboratory Spesies

The regional distribution of rats, guinea pigs,rabbits and mice was measure by high-performance liquid chromatography (HPLC) with electrochemical detection (ED). The catecholamines were adsorbed onto alumina and then eluted with 0.5 m hydrochloric acid and assayed by HPLC-ED. The lower limit of detection was about 0.03 -0.05 pmol. The predominant catecholamine was norepinephrine (NE), and dopamine (DA) was second. Epinephrine was not always detected. NE and DA were found in higher concentrations in the atria than in the ventricles. In the mouse NE was found in higher concentrations in the left heart region than in the right heart region. And DA was found in higher concentrations in the left atrium than in the right atrium. In order animals NE and DA were found in higher concentrations in the right heart region than in the left heart region. Thus, in studies of myocaedial catecholamines in addition to catecholamine metabolism it may be useful to consider catecholamine concentrations by heart regions and by species.

Catecholamine

Heart Tssue

High-Performance Liquid Chromatography with Electrochemical Detection