Immunologic Measurement of DNA Synthesis Rate in Individual Mouse L Cells

Quantitative analysis of IgG, IgA and IgM in serum and sputum from patients with chronic obstructive lung desease was carried out by radial immunodiffusion method. The concentration of IgE in the sera and sputa was also determined by radioimmunosorbent test. The mean IgA level in the sputa showed no significant defference between the control, bronchial asthma, chronic bronchitis and pulmonary emphysema groups, but the sputam from patients with pulumonary emphysema had a tendency to give higher mean values of IgA. The mean value of IgE in the sputam was not significantly different among four groups, but the number of sputa with measurable IgE concentration was much greater in chronic obstructive lung desease group. There was no significant difference of the mean IgG level in the sputa between the four groups, but there was a tendency to increase the mean IgG level in the sputa from the bronchial asthma group. IgM was detectabel in a few samples. Results of the present study suggested that the increased IgA and IgE in sputa might play a role in causative and/or pathophysiological mechanism in cases of chronic obstructive lung disease.

anti-BUdR antibody

double thymidine block

DNA synthesis rate

mouse L cell