Clinical Evaluation of Adjunct Procedures in Aneurysmectomy of the Descending Thoracic Aorta

The effect of hydrocortisone on the mixed lymphocyte reaction (MLR) in human peripheral blood was studied by morphological and cytochemical techniques. Hydrocortisone markedly inhibited the lymphocyte transformation in mixed lymphocyte culture (MLC). The extent of the inhibition of blastogenesis depended on the concentration of hydrocortisone. Those concentrations of hydrocortisone that inhibited blastogenesis in MLC were significantly lower than those necessary to inhibit PHA induced blastogenesis. The inhibitory effect of hydrocortisone also depended on the interval of time at which it was added after the onset of the MLC. A substantial loss of the inhibitory action of hydrocortisone upon the MLR was seen when the steroid was removed by washing 30 minutes after the onset of the culture. The increase in Feulgen-DNA content of the lymphocytes was clearly observed in MLC. Such an increase of the DNA value was not observed in 7 day old MLC with hydrocortisone. Especially a population of cells which contain tetraploid level (4c) was considerably reduced.

adjunct procedures

vascular prosthesis bypass

partial left heart bypass

femoral vein to femoral artery bypass