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Nakamura Kazuyuki

Affiliate Master Yamaguchi University

Study of the Interaction Between NAD- and NADP- Dependent Dehydrogenases and Immobilzed Adenine Nucleotidesvby Means of Affinity Electrophoresis II. Determination of Dissociation Constants of NAD-and NADP-Dependent Dehydrogenases for 2'-AMP-and-5'-AMP…

山口医学 Volume 30 Issue 3 Page 259-270
published_at 1981-09
Title
Affinityelectrophoresisによる固定化アデニンヌクレオチド誘導体とNAD-及びNADP-依存性脱水素酵素との特異結合反応の解析 IINAD-及びNADP-依存性脱水素酵素の固定化2'-AMP及び5'-AMPに対する解離度定数の算出
Study of the Interaction Between NAD- and NADP- Dependent Dehydrogenases and Immobilzed Adenine Nucleotidesvby Means of Affinity Electrophoresis II. Determination of Dissociation Constants of NAD-and NADP-Dependent Dehydrogenases for 2'-AMP-and-5'-AMP…
Creators Nakamura Kazuyuki
Source Identifiers
The dissociation constants of NAD-and NADP- dependent dehydrogenases for 2'-AMP and 5'-AMP were determined by affinity ele-trophoresis using 2'-AMP-and 5'-AMP-acrylamide copolymers. From the dissocation constants, it was found that NADP-dependent dehydrogenase (NADP-dependent DH) had a much stronger affinity to the immobilized 2'-AMP than did NAD-dependent dehydrogenase (NAD-dependent DH). On the other hand, NADP-dependent DH had a much weaker affinity to immobilized 5'-AMP than did NAD-dependent DH. The effects of NADP+ and NAD+ on the binding of NADP-dependent DH to the immobilized 2'-AMP or the binding of NAD-dependent DH to the immobilized 5'-AMP were also investigated by affinity electrpphoresis…
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1981-09
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部