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フルテキストURLA020014000202.pdf ( 2.0MB ) 公開日 2010-04-16
タイトルAmino Acid Substitution in Hemoglobin M Akita
作成者Miyaji, Takaoki
Iuchi, Iwao
Karita, Kosaku
Ohba, Yuzo
Yamamoto, Kiyomi
Shibata, Susumu
作成者ヨミミヤジ, タカオキ
イウチ, イワオ
カリタ, コサク
オオバ, ユウゾウ
ヤマモト, キヨミ
シバタ, ススム
作成者所属山口大学医学部
内容記述(抄録等)A new variant of Hb M was discovered from a family of hereditary cyanosis living in Akita by combined use of conventional tests for Hb M including spectroscopy of methemoglobin type hemolysate, agar gel electrophoresis (pH 7.0) and Amberlite IRC 50 chromatography. The hemoglobin was caued Hb M_Akita. MetHb M_Akita was purified by starch block electrophoresis (pH 7.0) of the metHb hemolysate of patient and deprived of heme to get M_Akita globin. Mixture of β chains (P^M+P^A) was also purified by CMC chromatography in 8M urea of the globin (Hb M_Akita + Hb A) prepared from the patient's hemolysate. The globin and the β chain mixture were aminoethylated, digested, with trypsin and then fingerprinted. The fingerprint thus made revealed abnormal spots related to peptides βTp-10, and βTp-10, 11. These were eluted, and hydrolyzed with hydrochloric acid. The amino acid analysis of the acid hydrolysate has demonstrated that, in Hb M_Akita, the ninety-second residue of the β chain, His (β 92), is substituted for by tyrosine. This hemoglobin is therefore expressed by the formula α_2β_2^92Tyr. Hb M_Akita is identical with Hb M_Hyde Park which was detected from a negro patient in the United States several months earlier.
本文言語eng
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University Graduate School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
14
2
開始ページ181
終了ページ190
発行日1967-06
著者版/出版社版出版社版
リポジトリIDA020014000202
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020014000202