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タイトルComparative study and cDNA cloning of the flavoprotein subunit of mitochondrial complex II (succinate-ubiquinone oxidoreductase: fumarate reductase) from the dog heartworm, dirofilaria immitis
作成者Kuramochi, Toshiaki
Kita, Kiyoshi
Takamiya, Shinzaburo
Kojima, Somei
Hayasaki, Mineo
作成者ヨミクラモチ, トシアキ
キタ, キヨシ
タカミヤ, シンザブロウ
コジマ, ソメイ
ハヤサキ, ミネオ
作成者別表記早崎, 峯夫
作成者所属山口大学農学部
内容記述(抄録等)Mitochondrial complex II functions as a fumarate reductase (FRD), the reverse reaction of succinate dehydrogenase (SDH), and plays an important role in the anaerobic respiratory chain of parasitic helminths. In this study, complex II from the dog heartworm, Dirofilaria immitis adult, which is thought to act as a homolactatic fermenter, was examined in terms of its enzymatic features and primary structure in order to investigate the possible role of mitochondria in this filaria. Mitochondria from D. immitis adult showed high FRD activity when the enzymatic assay was performed using methylviologen as an artificial electron donor. The ratio of SDH to FRD in D. immitis was comparable to that in Ascaris suum adult, which is known to have an anaerobic mitochondrial respiratory chain with a high FRD activity of complex II. The FRD activity of D. immitis mitochondria was inhibited by the sulfhydryl reagent N-ethylmaleimide (NEM), while that of A. suum complex II was resistant to this inhibitor. The presence of the flavoprotein (Fp) subunit, which contains the substrate binding active site, was confirmed in D. immitis mitochondria by immunoblotting using a monoclonal antibody against the A. suum Fp subunit. By homology probing with the polymerase chain reaction, the entire cDNA for the D. immitis adult Fp was cloned and sequenced. The deduced amino acid sequence showed significant homology to that of A. suum and other mitochondrial Fps, in contrast to much less similarity to bacterial FRD, even though the D. immitis complex II showed high FRD activity. These results are the first indication of the presence of a functional complex II in D. immitis mitochondria.
本文言語eng
著者キーワードActive site
cDNA sequence
Dirofilaria immitis
Flavoprotein subunit Fumarate reductase
Mitochondrial complex II
Succinate dehydrogenase
Sulfhydryl inhibition
資料タイプtext
出版者Pergamon Press
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN1096-4959
NCIDAA11045017
掲載誌名Comparative biochemistry and physiology. B, Biochemistry & molecular biology : an international journal
111
3
開始ページ491
終了ページ502
発行日1995
DOIinfo:doi/10.1016/0305-0491(95)00022-Z
関連情報URL(IsPartOf)http://www.sciencedirect.com/science/journal/10964959
著者版/出版社版その他
リポジトリID2008010378
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2008010378