Japanese | English

トップページへ戻る

詳細

   
フルテキストURLフルテキストリンクなし
タイトルPurification of GVBD-inducing protein from the ciliate tetrahymena thermophila
作成者Sugii, Manabu
Fujishima, Masahiro
作成者ヨミスギイ, マナブ
フジシマ, マサヒロ
作成者別表記杉井, 学
藤島, 政博
作成者所属山口大学大学情報機構
山口大学大学院理工学研究科(理学)
内容記述(抄録等)Germinal-vesicle-breakdown (GVBD) was induced if a 132,000-g supernatant of Tetrahymena thermophila homogenates was injected into Xenopus oocytes. Using this induction of GVBD as a bioassay system, a GVBD-inducing substance was purified from the Tetrahymena by ultra-filtration, liquid chromatography, and electroelution from a band on native-PAGE gel. Proteins eluted from the single band on the native-PAGE gel induced GVBD in the absence of oocyte protein synthesis. This band resolved into two bands on SDS-PAGE: 60 and 1 12 kDa. The 60 kDa protein was the active fraction inducing GVBD. Immunoprecipitation of the 60 kDa protein prevented the GVBD-inducing activity, supporting the conclusion that the 60 kDa protein is the GVBD-inducing substance. An immunoblot with anti-60 kDa monoclonal antibody and PSTAIR antibody showed that p 13^<sue1>-beads could remove cdc2 homologues from T. thermophila supernatant but could not remove the GVBD-inducing activity. The 60-kDa protein appeared at the same time as micronuclear division and disappeared at the beginning of the macronuclear division during synchronous cell division. The cyclic appearance of the 60-kDa protein in the T. thermophila cell cycle suggests that this protein has a cell cycle function.
本文言語eng
著者キーワードcdc2 homologue
cell cycle
M-phase promoting factor
PSTAIR antibody
p 13^<sue1>-beads
Xenopus oocytes
資料タイプtext
出版者Blackwell
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN1066-5234
NCIDAA10895978
掲載誌名The journal of eukaryotic microbiology
48
4
開始ページ414
終了ページ424
発行日2001-07
DOIinfo:doi/10.1111/j.1550-7408.2001.tb00174.x
関連情報URL(IsPartOf)http://www.blackwell-synergy.com/loi/jeu
著者版/出版社版その他
リポジトリID2008010120
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2008010120