| フルテキストURL | フルテキストリンクなし |
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| タイトル | Purification of GVBD-inducing protein from the ciliate tetrahymena thermophila |
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| 作成者 | Sugii, Manabu
Fujishima, Masahiro
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| 作成者ヨミ | スギイ, マナブ
フジシマ, マサヒロ
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| 作成者別表記 | 杉井, 学
藤島, 政博
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| 作成者所属 | 山口大学大学情報機構
山口大学大学院理工学研究科（理学）
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| 内容記述（抄録等） | Germinal-vesicle-breakdown (GVBD) was induced if a 132,000-g supernatant of Tetrahymena thermophila homogenates was injected into Xenopus oocytes. Using this induction of GVBD as a bioassay system, a GVBD-inducing substance was purified from the Tetrahymena by ultra-filtration, liquid chromatography, and electroelution from a band on native-PAGE gel. Proteins eluted from the single band on the native-PAGE gel induced GVBD in the absence of oocyte protein synthesis. This band resolved into two bands on SDS-PAGE: 60 and 1 12 kDa. The 60 kDa protein was the active fraction inducing GVBD. Immunoprecipitation of the 60 kDa protein prevented the GVBD-inducing activity, supporting the conclusion that the 60 kDa protein is the GVBD-inducing substance. An immunoblot with anti-60 kDa monoclonal antibody and PSTAIR antibody showed that p 13^<sue1>-beads could remove cdc2 homologues from T. thermophila supernatant but could not remove the GVBD-inducing activity. The 60-kDa protein appeared at the same time as micronuclear division and disappeared at the beginning of the macronuclear division during synchronous cell division. The cyclic appearance of the 60-kDa protein in the T. thermophila cell cycle suggests that this protein has a cell cycle function.
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| 本文言語 | eng
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| 著者キーワード | cdc2 homologue
cell cycle
M-phase promoting factor
PSTAIR antibody
p 13^<sue1>-beads
Xenopus oocytes
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| 資料タイプ | text |
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| 出版者 | Blackwell
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| NII資料タイプ | 学術雑誌論文 |
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| 査読の有無 | 査読あり |
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| ISSN | 1066-5234
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| NCID | AA10895978
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| 掲載誌名 | The journal of eukaryotic microbiology |
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| 巻 | 48 |
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| 号 | 4 |
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| 開始ページ | 414 |
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| 終了ページ | 424 |
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| 発行日 | 2001-07 |
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| DOI | info:doi/10.1111/j.1550-7408.2001.tb00174.x |
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| 関連情報URL(IsPartOf) | http://www.blackwell-synergy.com/loi/jeu
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| 著者版/出版社版 | その他 |
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| リポジトリID | 2008010120 |
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| 地域区分 | 山口大学
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| URI | http://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2008010120 |
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