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タイトルThe PCR-based detection of babesia gibsoni infection in dogs (German shepherds) reared in South Korea
作成者Song, K. H.
Kim, D. H.
Hayasaki, Mineo
作成者ヨミハヤサキ, ミネオ
作成者別表記早崎, 峯夫
作成者所属山口大学農学部
内容記述(抄録等)German-shepherd dogs from the South Korean provinces of Gangwon and Gyunggi were used in a study exploring both the detection of Babesia gibsoni infection, by use of a PCR-based assay, and the nucleotide sequences of part of the P18 gene of the parasite. Nine (1.8%) of the 501 dogs checked were found PCR-positive. Although the prevalence of the infection appeared slightly higher in the male dogs than in the female (2% υ. 1.7%), and in the dogs aged <3 years than in the older animals (2.7% υ. 1.1%), these differences were not statistically significant. In addition, the prevalences of infection among the dogs from Gangwon (in the east of the country) and those from Gyunggi (in the west) appeared similar. All nine PCR-positive dogs were confirmed to have B. gibsoni infection by the microscopical examination of thin bloodsmears, which revealed that 0.1%-3% (mean=1.5%) of the dog's erythrocytes harboured the parasite. The nucleotide sequences of the P18 gene of B. gibsoni from each of the PCR-positive dogs were identical to each other and to the corresponding sequence - recorded under accession number AB053292 in the DNA Data Bank of Japan (DDBJ) - previously determined for isolates of B. gibsoni from Japanese dogs. Although the PCR products sequenced in the present study represent only a part of the P18 gene of B. gibsoni, it seems that South Korean and Japanese dogs carry a similar strain of B. gibsoni. These results may help to clarify the phylogenetic position of the B. gibsoni found in South Korea.
本文言語eng
資料タイプtext
出版者Maney Publishing
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN0003-4983
NCIDAA00533245
掲載誌名Annals of tropical medicine and parasitology
98
2
開始ページ149
終了ページ153
発行日2004
DOIinfo:doi/10.1179/000349804225003226
関連情報URL(IsPartOf)http://www.ingentaconnect.com/content/maney/atmp;jsessionid=obnu7cpzsqtc.alice
著者版/出版社版その他
リポジトリID2008010388
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2008010388