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フルテキストURL2008010280.pdf ( 977.1KB ) 公開日 2010-04-16
タイトルAntigenic identification of excretory-secretory products of adult Dirofilaria immitis
作成者Kaneko, Hideto
Hayasaki, Mineo
Ohishi, Isamu
作成者ヨミカネコ, ヒデト
ハヤサキ, ミネオ
オオイシ, イサム
作成者別表記早崎, 峯夫
作成者所属山口大学農学部
内容記述(抄録等)Excretory-secretory (ES) products collected from adult Dirofilaria immitis cultured in vitro were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. ES products of male (M-ES) and female (F-ES) worms were separated into 16 and 21bands by SDS-PAGE with Coomassie blue and silver staining, respectively. The antigenic bands were then analyzed by immunoblotting, using pooled sera from dogs that had naturally contracted D. immitis. Sera from dogs with microfilaremic infection showed 7 bands in M-ES and 10 bands in F-ES, while those from dogs with occult infection revealed 3 bands in M-ES and 10 bands in F-ES. Among these bands, those of 14, 18, 21, 22, 29, and 32 kilodaltons (Kd) were common tho M-ES and F-ES, those of 39 and 44 Kd were specific to M-ES, and those of 20, 38, 43, 53, 63, 90, 110, 125 and 136 Kd were specific to F-ES.
本文言語eng
著者キーワードDirofilaria immitis
dog
excretory-secretory product
immunoblotting
資料タイプtext
ファイル形式application/pdf
出版者日本獣医学会
出版者ヨミニホン ジュウイ ガッカイ
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN0021-5295
NCIDAA10521639
掲載誌名Japanese journal of veterinary science
52
5
開始ページ995
終了ページ1000
発行日1990
関連情報URL(IsPartOf)http://www.jstage.jst.go.jp/browse/jvms/-char/ja
Dirofilaria immitis infection and dirofilariosis : an animal model of human filariosis
著者版/出版社版出版社版
リポジトリID2008010280
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2008010280