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タイトルラットにおける小脳半球虚血および再開通モデルの作製
タイトルヨミラット ニ オケル ショウノウ ハンキュウ キョケツ オヨビ サイカイツウ モデル ノ サクセイ
タイトル別表記A new experimental recirculation model for cerebellar hemisheric ischemia in the rat
作成者城山, 雄二郎
山下, 哲男
阿美古, 征生
青木, 秀夫
作成者ヨミシロヤマ, ユウジロウ
ヤマシタ, テツオ
アビコ, セイショウ
アオキ, ヒデオ
作成者別表記Shiroyama, Yujiro
Yamashita, Tetsuo
Abiko, Seisho
Aoki, Hideo
作成者所属山口大学医学部
内容記述(抄録等)Recently, both permanent and transient experimantal ischemic models have been reported using various laboratory animals. For the posterior fossa, there have been only a few ischemic models. This report describes a surgical approach in producing a new experimental recirculation model for cerebellar hemispheric ischemia in the rat. 1) At first, we made cast phenolic resin models of the vascular structure of the rat's brain. Using this model, we made an embolus by coating a 6-0 monofilament nylon thread with silicon rubber latex. 2) Adult male Wistar rats (300-350g) were anesthetized with pentobarbital sodium (40mg/kg). Through the median neck incision, cervical vertebral (C1-2 portion) were exposed. The transverse process of the second vertebra was carefully removed and the vertebral artery exposed for 3-4mm at the C1-2 level. Then, superior cerebellar artery (SCA) was occluded with the prepared embolic thread which was inserted 19mm into the vertebral artery. Next, the following studies were performed. (a) In one group, carbon black was perfused after 2 hours of ischemia. (b) In the other group, circulation in the superior cerebellar artery was restored by taking out the embolic thread after 6 hours of ischemia. Evans blue dye was then intravenously injected, and 2 hours agter recirculation, tissue was fixed by perfusion of formaline into the thoracic aorta. In all animals, gross brain was examined. In most of the SCA occluded animals, there was pallor of the cerebellar hemishper indicating failure of the carbon black perfusion. In the recirculation group, there was blue indicating damage of blood brain barrierd by Evans blue stain. These lesions revealed almost the same extension in both groups and were restricted to the cerebellar hemispher which, in the rat, is normally supplied by the superior cerebellar artery. These changes were detected in the cerebellar hemishper of 8 out of 19 rats. Our results appear to indicate that this model may be useful in the evaluation of pathophysiological states of cerebellar hemispheric ischemia and recirculation.
本文言語jpn
主題医学
資料タイプtext
出版者山口大学医学会
出版者ヨミヤマグチ ダイガク イガッカイ
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN0513-1731
NCIDAN00243156
学内刊行物(紀要等)山口医学
掲載誌名山口医学
37
5
開始ページ355
終了ページ361
発行日1988-10
著者版/出版社版その他
リポジトリIDB030037000506
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/B030037000506