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タイトル扁平上皮癌関連抗原TA-4のin vitro合成とパルスラベル法による解析
タイトルヨミヘンペイ ジョウヒ ガン カンレン コウゲン TA-4 ノ in vitro ゴウセイ ト パルスラベルホウ ニヨル カイセキ
タイトル別表記Pluse-Labeling studies of tumor-antigen TA-4 in squamous cell carcinoma cells
作成者橋本, 恭治
作成者ヨミハシモト, キョウジ
作成者別表記Hashimoto, Kyoji
作成者所属山口大学医学部
内容記述(抄録等)A tumor antigen TA-4 of squamous cell carcinoma can be devided into two subgroups (acidic TA-4 with pI of less than 6.25 and neutral TA-4 with pI of 6.25 or greater) by isoelectric focusing method (IFF), and acidic TA-4 is particularly relating to the malignant cells. The present study demonstrates the production of acidic TA-4 by in vitro cell line system. SKG III a cells, an established cell line of squamous cell carcinoma, were labeled with ^3H-labeled leucine for 3 minutes, and IFF patterns of radiolabeled TA-4 subfractions were chased at different intervals of time after the label. Immediately after the label, the radioactivity appeared only in acidic TA-4, which remains for up to 2 hours and disappeared 24 hours after the label. Small amount of radiolabeled neutral TA-4 was produced when the cells were incubated with ^3H-leucine for 24 hours. The cells were also incubated with several radiolabeled sugar, including ^3H-mannose, ^3H-glucosamie, or ^3H-galactose, for 24 hours, resulting in the incorpolation of the radioactivity into acidic TA-4. These results indicated that acidic TA-4 was synthesized in a different manner from neutral TA-4, and that acidic TA-4 contained some sugar chains.
本文言語jpn
主題医学
資料タイプtext
出版者山口大学医学会
出版者ヨミヤマグチ ダイガク イガッカイ
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN0513-1731
NCIDAN00243156
学内刊行物(紀要等)山口医学
掲載誌名山口医学
36
5
開始ページ303
終了ページ308
発行日1987-10
著者版/出版社版その他
リポジトリIDB030036000505
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/B030036000505