Japanese | English

トップページへ戻る

詳細

   
フルテキストURL2009010007.pdf ( 1.4MB ) 公開日 2010-05-07
タイトルProduction and characterization of alien chromosome additions in shallot (Allium cepa L. Aggregatum group) carrying extra chromosome(s) of Japanese bunching onion (A. fistulosum L.)
作成者Hang, Tran Thi Minh
Shigyo, Masayoshi
Yamauchi, Naoki
Tashiro, Yosuke
作成者ヨミHang, Tran Thi Minh
シギョウ, マサヨシ
ヤマウチ, ナオキ
タシロ, ヨウスケ
作成者別表記執行, 正義
作成者所属山口大学農学部
内容記述(抄録等)First and second backcrosses of amphidiploid hybrids (2n = 4x = 32, genomes AAFF) between shallot (Allium cepa Aggregatum group) and A. fistulosum were conducted to produce A. cepa - A. fistulosum alien addition lines. When shallot (A. cepa Aggregatum group) was used as a pollinator, the amphidiploids and allotriploids set germinable BC1 and BC2 seeds, respectively. The 237 BC1 plants mainly consisted of 170 allotriploids (2n = 3x = 24, AAF) and 42 hypo-allotriploids possessing 23 chromosomes, i.e., single-alien deletions (2n = 3x-1 = 23, AAF-nF). The single-alien deletions in the BC1 progeny showed dwarfing characteristics and were discriminated from the allotriploids (2n = 24) and hyper-allotriploids (2n = 25) by means of flow cytometric analysis. The chromosome numbers of 46 BC2 seedlings varied from 16 to 24. Eight monosomic additions (2n = 2x+1 = 17, AA+nF) and 20 single-alien deletions were found in these BC2 seedlings. Consequently, six kinds of A. cepa - A. fistulosum alien chromosome additions possessing different chromosome numbers (2n = 17, 18, 20, 21, 22, 23) were recognized in the BC1 and BC2 populations. A total of 79 aneuploids, including 62 single-alien deletions, were analyzed by a chromosome 6F-specific isozyme marker (Got-2) in order to recognize its existence in their chromosome complements. This analysis revealed that two out of 62 single-alien deletions did not possess 6F. One (AAF-6F) out of the possible eight single-alien deletions could be identified at first. The present study is a first step toward the development of a useful tool, such as a complete set of eight different single-alien deletions, for the rapid chromosomal assignment of genes and genetic markers in A. fistulosum.
本文言語eng
著者キーワードalien addition line
Allium cepa
Allium fistulosum
flow cytometry
isozyme
資料タイプtext
ファイル形式application/pdf
出版者日本遺伝学会
出版者ヨミニホン イデン ガッカイ
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN1341-7568
NCIDAA11077421
掲載誌名Genes & genetic systems
79
5
開始ページ263
終了ページ269
発行日2004
DOIinfo:doi/10.1266/ggs.79.263
関連情報URL(IsPartOf)http://www.jstage.jst.go.jp/browse/ggs/_vols
著者版/出版社版出版社版
リポジトリID2009010007
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2009010007