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タイトルカルモジュリン阻害剤によるヒト・リンパ球活性化反応の阻害とその加齢変化
タイトルヨミカルモジュリン ソガイザイ ニヨル ヒト リンパキュウ カッセイカ ハンノウ ノ ソガイ トソノ カレイ ヘンカ
タイトル別表記Calmodulin Antagonist, W-7 Inhibits the Mitogen-induced Proliferation in Peripheral Human Lymphocytes, Reflecting Alterations Associated with Aging
作成者開地, 泰介
作成者ヨミカイチ, ヤススケ
作成者別表記Kaichi, Yasusuke
作成者所属山口大学医学部
内容記述(抄録等)O investigate whether calmodulin can be related to the mitogen-induced proliferation by phytohemagglutinin-P (PHA-P) in peripheral lymphocytes, we studied the effect of calmodulin antagonist, N-(6-aminohexyl)-5-chloro-l-naphthalenesulfonamide hydrochloride (W-7) , on the mitogenic reaction. Healty human adults both males and females, age range 40-68) were examined. Under the stimulation with the mitogen PHA-P, lymphocytes were incubated for 72 hrs at 37℃ , with W-7 of 0, 2.5, 5, 10, 25 and 50μM. The effects of W-7 were determined by the inhibition of DNA synthesis, measuring the incorporation of [3^H]thymidine ([3^H]dT), in the culture medium with and without W-7. In all of the 36 subjects, W-7 exhibited the inhibition of [3^H]dT incorporation. These findings suggest that W-7-inhibits the PHA-mitogenic reaction, and that calmodulin is related to the initiation of the mitogen-induced proliferation. Furthermore, for all subjects, a positive correlation (N=36, r=0.457, ρ<0.01) was found between the age and inhibition ratio with W-7 at 10μM. These findings suggest that the marked increase in inhibition ratio with W-7 serves as a useful marker, reflecting the alterations associated with aging.
本文言語jpn
著者キーワードカルモジュリン
W-7
リンパ球
PHA
加齢の指標
主題医学
資料タイプtext
出版者山口大学医学会
出版者ヨミヤマグチ ダイガク イガッカイ
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN0513-1731
NCIDAN00243156
学内刊行物(紀要等)山口医学
掲載誌名山口医学
43
6
開始ページ383
終了ページ391
発行日1994-12
著者版/出版社版その他
リポジトリIDB030043000602
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/B030043000602