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フルテキストURL2009010006.pdf ( 969.8KB ) 公開日 2010-05-07
タイトルIdentification of alien chromosomes in a series of Allium fistulosum - A. cepa monosomic addition lines by means of genomic in situ hybridization
作成者Shigyo, Masayoshi
Imamura, Kenzi
Iino, Mitsuyasu
Yamashita, Ken-ichiro
Tashiro, Yosuke
作成者ヨミシギョウ, マサヨシ
イマムラ, ケンジ
イイノ, ミツヤス
ヤマシタ, ケンイチロウ
タシロ, ヨウスケ
作成者別表記執行, 正義
作成者所属山口大学農学部
内容記述(抄録等)Genomic in situ hybridization (GISH) was used to identify alien chromosomes in a series of eight different Allium fistulosum - A. cepa monosomic addition lines. Biotin-labeled total genomic DNA from shallot (A. cepa L. Aggregatum group) was used as a probe together with an excess amount of unlabeled blocking DNA from the recipient plant, Japanese bunching onion (A. fistulosum L.). Probe hybridization sites were detected by FITC-conjugated avidin and anti-avidin antibody using an epifluorescence microscope. In each mitotic metaphase cell of all the eight types of monosomic addition lines, the alien chromosomes were successfully discriminated from other 16 A. fistulosum chromosomes. Furthermore, no clear exchanges of chromosome segments between A. cepa and A. fistulosum were observed. This finding indicates that in each addition line an entire (unrecombined) A. cepa chromosome is present in an integral diploid background of A. fistulosum.
本文言語eng
資料タイプtext
ファイル形式application/pdf
出版者日本遺伝学会
出版者ヨミニホン イデン ガッカイ
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN1341-7568
NCIDAA11077421
掲載誌名Genes & genetic systems
73
5
開始ページ311
終了ページ315
発行日1998
DOIinfo:doi/10.1266/ggs.73.311
関連情報URL(IsPartOf)http://www.jstage.jst.go.jp/browse/ggs/_vols
著者版/出版社版出版社版
リポジトリID2009010006
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2009010006