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タイトル臓器資料からの有機リン系農薬の分離同定とアセチルコリンエステラーゼ活性値測定
タイトルヨミゾウキ イリョウ カラ ノ ユウキ リンケイ ノウヤク ノ ブンリ ドウテイ ト アセチルコリン エステラーゼ カッセイチ ソクテイ
タイトル別表記On the Isolation and Identification of Organophosphorus Pesticide from Various Specimens of a Female Corpse and the Assay of Acetylcholinesterase Activity in the Blood
作成者菅原, 憲典
古野, 潤治
入沢, 淑人
作成者ヨミスガワラ, ノリスケ
フルノ, ジュンジ
イライザワ, ヨシト
作成者別表記Sugawara, Norisuke
Furuno, Junji
Irizawa, Yoshito
作成者所属山口大学医学部
内容記述(抄録等)Recently, the authors have experienced the autopsy of a female victim who died of organophosphorus pesticide intoxication. Therefore, for the purpose of clarifying the dead cause, we carried out the separation and identification of organophosphorus pesticide from the specimens such as the stomach contents, cardiac blood and urine of this cadaver and further the measurement of acetylcholinesterase activity in the blood. The results were as follows. 1. Acetylcholinesterase activity in the serum and erythrocytes. The serum and erythrocytes were separated by centrifugation for 5 minutes at 3000rpm and subjected to the assay of acetylcholinesterase activity on the basis of Pilz's method. Acetylcholinesterase activities were 0.312μAq in the erythrocytes and 0.078μAq in the serum, respectively. These values abruptly lowered in comparison with those in the serum and erythrocytes of healthy human adults and the inhibition of acetylcholinesterase activity was clearly observed. 2. Separation and identification of organophosphorus pesticide Unknown organophosphorus pesticide was first extracted from the stomach contents, cardiac blood and urine with ethanol according to Stas-Ott's method and then the crude materials purified by use of both the silicagel column and thin-layer chromatographies. Subsequently the qualitative analysis of organophosphorua pesticide was undertaken by means of ρ-nitrophenol and Averell-Norris color reactiton, ultraviolet absorption spectrophotometry, infrared absorption spectrophotometry, thin-layer chromatography and gas chromatography. As a result, the organophosphorus pesticide extracted from various specimens of this cadaver was identified as malation.
本文言語jpn
主題医学
資料タイプtext
出版者山口大学医学会
出版者ヨミヤマグチ ダイガク イガッカイ
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN0513-1731
NCIDAN00243156
学内刊行物(紀要等)山口医学
掲載誌名山口医学
31
6
開始ページ695
終了ページ700
発行日1982-12
著者版/出版社版その他
リポジトリIDB030031000614
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/B030031000614