Japanese | English

トップページへ戻る

詳細

   
フルテキストURLフルテキストリンクなし
タイトル増殖関連抗原の発言とS期移行との関連性に関する実験的研究
タイトルヨミゾウショク カンレン コウゲン ノ ハツゲン ト Sキ イコウ トノ カンレンセイ ニ カンスル ジッケンテキ ケンキュウ
タイトル別表記n Experimental Research on the Relation between the Expression of Proliferation Associated Antigens and the Transition from Quiescent Phase to S-phase of Cell Cycle
作成者森本, 宏志
作成者ヨミモリモト, ヒロシ
作成者別表記Morimoto, Hiroshi
作成者所属山口大学医学部
内容記述(抄録等)PCNA is an auxiliary protein of DNA polymerase d, and synthesized during late Gl to S phases in proliferating cells. The cells expressing Ki-67 reactive antigen, another proliferation associated antigen are supposed to be cycling cells. Correlations between positive rate of these antigens and S phase ratio are often discussed, but the relations between expression of these antigens and the transition to S phase are still controversial. Quiescent cells need to express PCNA to enter the proliferating phase, but all cell expressing PCNA do not necessarily enter the S phase. It is true of Ki-67 reactive antigen positive cells, too. In this study, the order of the expression of PCNA and Ki-67 weather arnigen were observed during the transition from quiescent phase to proliferating phase of cell cycle using normal human lymphocytes stimulated by mitogenic agent of PHA (bacto-phytohemagglutinin M). As a result, at 24 hours after PHA stimulation, the PCNA positive rate began to inerease. After another 12 hours, Ki-67 reactive antigen positive rate, and at 48hours after stimulation, BrdU labeling index also began to increase. The correlations between the positive rates of PCNA and Ki-67 reactive antigen, PCNA and BrdU in human tumor tissues were also examined using 15 clinical cases. The result shows that all have significant positive correlation (PCNA:BrdU, r=0.73, p<0.05, Ki-67 reactive antigen:BrdU, r=0.82, p<0.01, PCNA:Ki-67 reactive antigen, r=0.92, p<0.01). There are some differences among the correlations, which may be caused by the order of expression of these proliferation associated antigens.
本文言語jpn
著者キーワードPCNA
BrdU
Ki-67
PHA刺激リンパ球
細胞周期
主題医学
資料タイプtext
出版者山口大学医学会
出版者ヨミヤマグチ ダイガク イガッカイ
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN0513-1731
NCIDAN00243156
学内刊行物(紀要等)山口医学
掲載誌名山口医学
44
2
開始ページ105
終了ページ111
発行日1995-04
著者版/出版社版その他
リポジトリIDB030044000206
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/B030044000206