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タイトル白血球ペルオキシダーゼ反応の検討
タイトルヨミハッケッキュウ ペルオキシダーゼ ハンノウ ノ ケントウ
タイトル別表記Some Observations on Peroxydase Reaction of Leukocytes
作成者中野, 保
作成者ヨミナカノ, タモツ
作成者別表記Nakano, Tamotsu
作成者所属山口大学医学部
内容記述(抄録等)The improved method of peroxydase reaction for human leukocyte analysis was studied comparing with the recommended clinical laboratory methods. This new method ”Benzidine-Zinc Method” differs from others in the pretreatment with ZnSO_4 solution, although it principally depends on the oxydation of benzidine by the peroxyde-peroxydase system. The main procedures of this method are following in order, fixation with 10% formalin-alcohol, Giemsa staining, 1% ZnSO_4 treatment and the benzidine-peroxide reaction lastly. The specificity of this reaction to leukocyte peroxydase was confirmed by careful comparison with the results of Sato-Sekitani's and Mc Junkin's methods. It is found that this method is more excellent at three points. First, the reaction products in blood cells are observable very clearly, that is intense blue in neutrophils, yellowish brown in acidophils and faint blue in monocytes. Secondly, identification of blood cell types is very easy according to per-staining wiith Giemsa solution. Thirdly , red corpuscles and blood platatelets are well preserved, and the pseudo-peroxydase reaction in red cells is negrigible. This method was applied to some Patients with leukemia and others, and the results obtained were discussed. Thus this ”Benzidine-Zinc Method” of peroxydase reaction seems to be reliable and excellent for clinical laboratory use.
本文言語jpn
主題医学
資料タイプtext
出版者山口大学医学会
出版者ヨミヤマグチ ダイガク イガッカイ
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN0513-1731
NCIDAN00243156
学内刊行物(紀要等)山口医学
掲載誌名山口医学
20
1
開始ページ11
終了ページ18
発行日1971-03
著者版/出版社版その他
リポジトリIDB030020000102
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/B030020000102