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タイトルβ-lactam系抗生物質のレプトスピラ細胞に及ぼす形態学的影響
タイトルヨミβ-lactam ケイ コウセイ ブッシツ ノ レプトスピラ サイボウ ニ オヨボス ケイタイガクテキ エイキョウ
タイトル別表記Morphological Effects of β-lactam Antibiotics on the Leptospira Cells
作成者渡辺, 和彦
作成者ヨミワタナベ, カズヒコ
作成者別表記Watanabe, Kazuhiko
作成者所属山口大学医学部
内容記述(抄録等)The morphological effects of β-lactam antibiotics, such as cefmetazole (CMZ), cephalexin (CEX) and penicillin-G (PC-G), on the cells of Leptospira canicola were investigated under electron microscopy. The efficacious concentrations of the antibiotics were decided to be 0.1μg/ml and 1.0μg/ml of CMZ, 1.0μg/ml and 10.0μg/ml of CEX, and 0.1u and 1.0u/ml of PC-G, respectively. Specimens were prepared from the cultures of leptospira cells with mass inoculation in Korthof's media, is containing the drugs with above concentrations, and incubated at 30℃ for 12 and 48hrs (CEX exceptional, for 4 days). The former incubation time corresponds to the logarithmic phase and the latter to the reducing phase on the growth curves. No remarkable difference could be observed in the morphological effects of cells among these drugs, however, much differences in the damages could be seen between two incubation times. The most definite effects were the subterminal ghost formation at one pole of the main body and appearance of the spherical body (SB) of swelling type in the 12 hours incubation. Formation of the whole cell ghost and of SB (of rolling type) could be seen in the 48 hours incubation. Through the process of these two different times of incubation, several types of intermediate form of SB were seen. From these morphological effects by the treatment of the antibiotics, the fundamental organization of the main body of leptospira cell and the formation process of SB with several types were discussed.
本文言語jpn
主題医学
資料タイプtext
出版者山口大学医学会
出版者ヨミヤマグチ ダイガク イガッカイ
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN0513-1731
NCIDAN00243156
学内刊行物(紀要等)山口医学
掲載誌名山口医学
32
3
開始ページ283
終了ページ314
発行日1983-06
著者版/出版社版その他
リポジトリIDB030032000306
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/B030032000306