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フルテキストURL2007020037.pdf ( 2.7MB ) 公開日 2010-09-28
タイトルApplication of a molecular method for the identification of a Gigaspora margarita isolate released in a field
作成者Yokoyama, Kazuhira
Tateishi, Takahiro
Saito, Masanori
Marumoto, Takuya
作成者ヨミヨコヤマ, カズヒラ
タテイシ, タカヒロ
サイトウ, マサノリ
マルモト, タクヤ
作成者別表記横山, 和平
立石, 貴浩
斎藤, 雅典
丸本, 卓哉
作成者所属山口大学農学部
内容記述(抄録等)A molecular technique for the identification of the Gigaspora margarita isolate CK based on the detection of a DNA sequence of 235 bp as its diagnostic marker was evaluated to investigate the survival and establishment of introduced arbuscular mycorrizal fungi (AMF) in a field ecosystem. In March 2001, roots and rhizosphere soil of Eragrostis curvula and Miscanthus sinensis were collected from the Mizunashi River at Mt. Fugendake (Nagasaki Prefecture, Japan), where plant seeds and AMF including G. margarita CK had been introduced for reforestation after the occurrence of repeated pyroclastic flows. We detected the marker sequence from DNA preparations of E. curvula roots and Gigaspora spores in the rhizosphere. This clearly showed that the isolate occurred at both hyphal and sporal stages. It was shown that the isolate survived and developed a life cycle in the revegetation area for 4 years. It was confirmed that the method was effective for tracing the isolate in samples collected from field ecosystems.
本文言語eng
著者キーワードdiagnostic DNA marker
field ecosystem
Gigaspora margarita
isolate-specific identification
reforestation
主題農学
資料タイプtext
ファイル形式application/pdf
出版者日本土壌肥料学会
出版者ヨミニホン ドジョウ ヒリョウ ガッカイ
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN0038-0768
NCIDAA00844314
掲載誌名Soil science and plant nutrition
51
1
開始ページ125
終了ページ128
発行日2005-02
DOIinfo:doi/10.1111/j.1747-0765.2005.tb00015.x
関連情報URL(HasVersion)http://ci.nii.ac.jp/naid/110004704728/
関連情報URL(IsPartOf)http://www.blackwellpublishing.com/journal.asp?ref=0038-0768&site=1
著者版/出版社版出版社版
リポジトリID2007020037
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2007020037