| フルテキストURL | A020048000304.pdf ( 4.1MB ) 公開日 2010-04-19
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| タイトル | A role of membrane type-1 matrix metalloproteinase in the regulation of vascular smooth muscle cell proliferation in vitro |
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| 作成者 | Hua, Zyang
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| 作成者所属 | 山口大学医学部
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| 内容記述（抄録等） | The purpose of this study was to assay changes in membrance type-1 matrix metalloproteinase (MT1-MMP) in vivo after vascular injury and to determine the influence of antisense MT1-MMP oligonucleotide on vascular smooth muscle cell proliferation and migration in vitro. mRNA expression of MT1-MMP was investigated in balloon-injured rat common carotid artery. The effect of antisense MT1-MMP oligonucleotide on activated by zymography. The influence of MT1-MMP antisense oligonucleotide on the proliferation and migration of cultured SMCs was measured by3-(4, 5-dimethylthiazol-2yl)2, 5-diphenyl tetrasodium bromine assay and a 24-well transwell cell culture chamber method. The expression of MT1-MMP mRNA was increased in injured arteries, reaching a peak increace at 4 days after inhibited slightly by antisense MT1-MMP oligonucletide. Antisense MT1-MMP oligonucleotide significantly inhibited SMC proliferation (p＜0.05) but had little effect on the migration of cultured SMCs. Expression of MT1-MMP mRNA increases following arterial injury. Antisense MT1-MMP oligonucleotide regulates SMC proliferation and may therefore constitune a featment for inhibition of intimal hyperplasia.
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| 本文言語 | eng
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| 著者キーワード | smooth muscle cell prolifeeration
migration
membrane type-1 matrix metalloproteinase
matrix metalloproteinase-2
antisense
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| 主題 | 医学
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| 資料タイプ | text |
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| ファイル形式 | application/pdf |
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| 出版者 | Yamaguchi University School of Medicine
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| NII資料タイプ | 紀要論文 |
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| ISSN | 0513-1812
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| NCID | AA00594272
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| 学内刊行物(紀要等) | The bulletin of the Yamaguchi Medical School |
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| 掲載誌名 | The bulletin of the Yamaguchi Medical School |
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| 掲載誌名別表記 | 山口医科大学紀要 |
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| 巻 | 48 |
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| 号 | 3-4 |
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| 開始ページ | 47 |
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| 終了ページ | 56 |
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| 発行日 | 2001-12 |
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| 著者版/出版社版 | 出版社版 |
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| リポジトリID | A020048000304 |
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| 地域区分 | 山口大学
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| URI | http://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020048000304 |
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