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フルテキストURLA020048000304.pdf ( 4.1MB ) 公開日 2010-04-19
タイトルA role of membrane type-1 matrix metalloproteinase in the regulation of vascular smooth muscle cell proliferation in vitro
作成者Hua, Zyang
作成者所属山口大学医学部
内容記述(抄録等)The purpose of this study was to assay changes in membrance type-1 matrix metalloproteinase (MT1-MMP) in vivo after vascular injury and to determine the influence of antisense MT1-MMP oligonucleotide on vascular smooth muscle cell proliferation and migration in vitro. mRNA expression of MT1-MMP was investigated in balloon-injured rat common carotid artery. The effect of antisense MT1-MMP oligonucleotide on activated by zymography. The influence of MT1-MMP antisense oligonucleotide on the proliferation and migration of cultured SMCs was measured by3-(4, 5-dimethylthiazol-2yl)2, 5-diphenyl tetrasodium bromine assay and a 24-well transwell cell culture chamber method. The expression of MT1-MMP mRNA was increased in injured arteries, reaching a peak increace at 4 days after inhibited slightly by antisense MT1-MMP oligonucletide. Antisense MT1-MMP oligonucleotide significantly inhibited SMC proliferation (p<0.05) but had little effect on the migration of cultured SMCs. Expression of MT1-MMP mRNA increases following arterial injury. Antisense MT1-MMP oligonucleotide regulates SMC proliferation and may therefore constitune a featment for inhibition of intimal hyperplasia.
本文言語eng
著者キーワードsmooth muscle cell prolifeeration
migration
membrane type-1 matrix metalloproteinase
matrix metalloproteinase-2
antisense
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
48
3-4
開始ページ47
終了ページ56
発行日2001-12
著者版/出版社版出版社版
リポジトリIDA020048000304
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020048000304