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タイトルOK-432皮下注刺激リンパ節単核球から誘導したLAK細胞による養子免疫治療法の基礎的検討
タイトルヨミOK-432 ヒカチュウ シゲキ リンパセツ タンカクキュウ カラ ユウドウ シタ LAK サイボウ ニヨル ヨウシ メンエキ チリョウホウ ノ キソテキ ケントウ
タイトル別表記Induction of lymphokine activated killer cells from mononuclear cells of lymph nodes stimulated with OK-432
作成者鈴木, 道成
作成者ヨミスズキ, ミチナリ
作成者別表記Suzuki, Michinari
作成者所属山口大学医学部
内容記述(抄録等)The present study examined the utility of lymphokine activated killer(LAK) cells derived from mononuclear cells(MNCs) of lymph nodes stimulated with subcutaneously injected OK-432. LAK cells were induced by 100JRU/ml of rIL-2 from the MNCs of the popliteal and axillary lymph nodes of mice stimulated with OK-432(OK-432 group) or saline alone(control group). The number of MNCs in the OK-432 group(17.7×10^6) significantly greater than that in the control group(3.9x10^6). The anti-YAC-1 activity and anti-EL-4 activity of LAK cells induced by 3 days of incubation in the OK-432 group were significantly higher than those in the control group. Phenotypic analysis demonstrated that the percentage of asialo GM1 positive lymphocytes of LAK cells in the OK-432 group was significantly higher than that in the control group, whereas the percentage of Thy1.2, L3/T4 and Ly2 positive cells in the OK-432 group was significantly lower than that in the control group. Both anti-YAC-1 and anti-EL-4 activities were strongly inhibited by treatment with anti-asialo GM1 serum plus complement. Thus, the effector cells of LAK cells were characterized as Thy1.2 and asialo GM1 positive cells. Winn's neutralization assay against Meth A tumor cells showed that the anti-tumor effect of LAK cells in the OK-432 group was not much greater than that in the control group. These results suggest that adoptive immunotherapy using LAK cells derived from MNCs of lymph nodes stimulated with subcutaneously injected OK-432 may be useful for cancer therapy.
本文言語jpn
著者キーワード局所リンパ節単核球
OK-432
interleukin-2
LAK細胞
養子免疫治療
主題医学
資料タイプtext
出版者山口大学医学会
出版者ヨミヤマグチ ダイガク イガッカイ
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN0513-1731
NCIDAN00243156
学内刊行物(紀要等)山口医学
掲載誌名山口医学
44
3
開始ページ145
終了ページ154
発行日1995-06
著者版/出版社版その他
リポジトリIDB030044000302
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/B030044000302