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フルテキストURL2007020169.pdf ( 2.8MB ) 公開日 2010-04-16
タイトルTransplantation of cryopreserved mouse, Chinese hamster, rabbit, Japanese monkey and rat ovaries into rat recipients
作成者Kagabu, Satosi
Umezu, Motoaki
作成者ヨミカガブ, サトシ
ウメズ, モトアキ
作成者別表記利部, 聰
梅津, 元昭
作成者所属山口大学農学部
内容記述(抄録等)Partial ovaries from mice, hamsters, rabbits, Japanese monkeys and rats have survived deep-freezing and returned to a normal morphological state after being thawed and transplanted into the rat uterine cavity. This report describes the ice-free cryopreservation of mouse and other ovaries at -196℃ by vitrification. The vitrification solution was based on the solutions reported by Rall & Fahy [16]. After ovaries had been exposed to the vitrification solution, they were frozen, with their suspending medium, by liquid nitrogen. After freezing, the ovaries were thawed in 37℃ water. The viability of the previously frozen ovarian tissue was tested by transplanting it into the uterine cavity of pseudopregnant rats. Seven days after transplantation, the ovaries were removed with the rat uterus, and stained with haematoxylin and eosin for histological examination. Survival of the frozen-thawed the ovaries in the rat uterine cavity demonstrates that these ovaries can tolerate exposure to osmotic dehydration and vitrification in a concentrated solution of cryoprotectant and are then immunologically acceptable to the uterine cavity.
本文言語eng
著者キーワードovary cryopreservation
ovary transplantation
rat uterine cavity
vitrification
資料タイプtext
ファイル形式application/pdf
出版者日本実験動物学会
出版者ヨミニホン ジッケン ドウブツ ガッカイ
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN1341-1357
NCIDAA11032321
掲載誌名Experimental animals
49
1
開始ページ17
終了ページ21
発行日2000
DOIinfo:doi/10.1538/expanim.49.17
関連情報URL(IsPartOf)http://www.jstage.jst.go.jp/browse/expanim/-char/ja/
著者版/出版社版出版社版
リポジトリID2007020169
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2007020169