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フルテキストURLA050067000101.pdf ( 1.6MB ) 公開日 2020-07-16
タイトルMIG and I309 accelerates proliferation of human bone marrow-derived mesenchymal stem cells
作成者Aibe, Yuki
Takami, Taro
Fujisawa, Koichi
Matsumoto, Toshihiko
Sakaida, Isao
作成者ヨミアイベ, ユウキ
タカミ, タロウ
フジサワ, コウイチ
マツモト, トシヒコ
サカイダ, イサオ
作成者別表記相部, 祐希
高見, 太郎
藤澤, 浩一
松本, 俊彦
坂井田, 功
作成者所属山口大学大学院医学系研究科(医学)
内容記述(抄録等)Background: Previous studies have shown that mesenchymal stem cells (MSCs) are an important source of somatic stem cells for regenerative medicine. Therefore, the establishment of efficient MSC culture methods to increase their therapeutic value is essential. Here, we focused on cytokines as accelerators of cell proliferation. Methods: We evaluated the effect of the supernatant—obtained from a co-culture of bone marrow-derived MSCs (BMSCs) and myeloid cells—on the proliferation of BMSCs. We then screened for cytokines present in high concentrations in the medium. Next, we added the identified cytokines to the culture medium in various combinations, and cultured BMSCs in this medium to evaluate the effects of cytokines on their proliferation and differentiation potential. Results: The medium obtained from the co-culture of BMSCs with myeloid cells stimulated BMSC proliferation. Nineteen cytokines were present in high concentrations in the medium. Out of the 19 cytokines, MIG and I309 stimulated BMSC proliferation. Furthermore, the culture medium supplemented with MIG and I309 maintained the differentiation potential of cultured BMSCs. Conclusions: MIG and I309 stimulate BMSC proliferation while maintaining their differentiation potential. These results may contribute to the establishment of more efficient MSC culture methods than those currently being used.
本文言語eng
著者キーワードmesenchymal stem cell
MIG
I309
cell proliferation
regenerative medicine
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
67
1-2
開始ページ1
終了ページ10
発行日2020
著者版/出版社版出版社版
リポジトリIDA050067000101
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A050067000101