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フルテキストURLA050061000301.pdf ( 1.7MB ) 公開日 2015-04-13
タイトルAnalysis of cerebrospinal fluid proteins reveals association of calbindin 1 concentrations with neurological outcomes in patients resuscitated from out-of-hospital cardiac arrest : a proteomics-based pilot study
作成者Akiyoshi, Yuki
Izumi, Tomonori
Oda, Yasutaka
Mizukami, Yoichi
Tsuruta, Ryosuke
Maekawa, Tsuyoshi
作成者ヨミアキヨシ, ユウキ
イズミ, トモノリ
オダ, ヤスタカ
ミズカミ, ヨウイチ
ツルタ, リョウスケ
マエカワ, ツヨシ
作成者別表記泉, 友則
小田, 泰崇
水上, 洋一
鶴田, 良介
前川, 剛志
作成者所属山口大学大学院医学系研究科(医学)
山口大学総合科学実験センター
内容記述(抄録等)The purpose of this study is to establish an analytical platform for identifying a major subset of cerebrospinal fluid (CSF) proteins in patients resuscitated from out-of-hospital cardiac arrest and evaluating their associations with neurological outcomes. Thirteen patients, who were resuscitated from cardiac arrest and survived for more than 48 hours, were enrolled and their CSF was obtained. Patients were classified into favorable (group F) and unfavorable (group U) outcomes based on the Glasgow Outcome Scale at 6 months after the return of spontaneous circulation.Control CSF was also obtained from 8 subjects with no obvious neurological disorders (group N). Protein identification was performed on one individual from each group by liquid chromatography-tandem mass spectrometry and verified on all 21 individuals by western blotting. In total, 107 proteins were identified in CSF. Of those, 40 proteins were identified only in the group U patients, including brain-specific proteins,such as enolase 2 and calbindin 1. The levels of calbindin 1 in individual CSF were significantly higher in group U than in group N or in group F, and associated with the Glasgow Outcome Scale scores. A combination of liquid chromatography-tandem mass spectrometry and western blotting is useful to analyze CSF from critical care unit patients.
本文言語eng
著者キーワードcerebrospinal fluid
proteomic analysis
cardiopulmonary arrest
calbindin 1
biomarker
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
61
3-4
開始ページ23
終了ページ35
発行日2014
著者版/出版社版出版社版
リポジトリIDA050061000301
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A050061000301