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フルテキストURLA050060000102.pdf ( 1.1MB ) 公開日 2014-02-21
タイトルSuperoxide stimulation enhances CXCR4 expression in heart muscle-derived stem cells via ASK1 activation
作成者Akashi, Shintaro
Miura, Toshiro
Shibuya, Masaki
Fukagawa, Yasuhiro
Oda, Takamasa
Nakamura, Takeshi
Ikeda, Yasuhiro
Matsuzaki, Masunori
Yano, Masafumi
作成者ヨミアカシ, シンタロウ
ミウラ, トシロウ
シブヤ, マサキ
フカガワ, ヤスヒロ
オダ, タカマサ
ナカムラ, タケシ
イケダ, ヤスヒロ
マツザキ, マスノリ
ヤノ, マサフミ
作成者別表記中村, 武史
矢野, 雅文
作成者所属山口大学医学部附属病院
山口大学大学院医学系研究科(医学)
内容記述(抄録等)Stem cell therapy for cardiac regeneration is hindered by poor homing to infarcted legion because of poor expression of homing factor, CXCR4, on the stem cell surface. A strategy for enhancing CXCR4 is promising to acceler- ate stem cell homing to infarcted lesion. Methods and Results: Heart muscle-derived Sca-1(+) cells (HDSCs) were isolated from normal mouse heart using surface antigen Sca-1. The expression of CXCR4 was determined by the fluorescence-activated cell sorting by labeling the cells with CXCR4 antibody with fluorescence. The expression of CXCR4 was enhanced by stimulation of hydrogen peroxide (0.1, 0.33, 1μM) for 24h. The Western blotting showed no significant increase in the protein level of CXCR4. The enhancement of CXCR4 can be attributed to translocation of CXCR4 from the cytosolic fraction to cell surface. H_2O_2 stimulation enhanced ASK1 p966 phosphory- lation. Conclusions: The expression of CXCR4 on HDSCs was augmented by H_2O_2 through the translocation from cytosolic to membrane surface through ASK1 phos-phorylation
本文言語eng
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
60
1-2
開始ページ11
終了ページ18
発行日2013
著者版/出版社版出版社版
リポジトリIDA050060000102
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A050060000102