| フルテキストURL | A050060000101.pdf ( 1.4MB ) 公開日 2014-02-21
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| タイトル | Phosphorylation of bad by protein Kinase-C activator can attenuate tongue muscle-derived stem cell death caused by oxidative stress |
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| 作成者 | Fukagawa, Yasuhiro
Miura, Toshiro
Shibuya, Masaki
Akashi, Shintaro
Oda, Takamasa
Nakamura, Takeshi
Matsuzaki, Masunori
Yano, Masafumi
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| 作成者ヨミ | フカガワ, ヤスヒロ
ミウラ, トシロウ
シブヤ, マサキ
アカシ, シンタロウ
オダ, タカマサ
ナカムラ, タケシ
マツザキ, マスノリ
ヤノ, マサフミ
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| 作成者別表記 | 中村, 武史
矢野, 雅文
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| 作成者所属 | 山口大学医学部附属病院
山口大学大学院医学系研究科（医学）
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| 内容記述（抄録等） | Background: Improving the survival of transplanted cells is a critical is- sue in the use of stem cell transplantation therapy for cardiovascular disease. Many transplanted cells are lost through apoptosis or necrosis triggered by hypoxia or superoxide. Methods and Results: Tongue muscle-derived Sca-1(+) cells were isolated from the mouse and cultured for 2 weeks and then exposed to hydrogen peroxide (H_2O_2;0.2 mM) for 24 h. The extent of cell death by either apoptosis or necrosis was mea-sured by fluorescence-activated cell sorting using annexin V and propidium iodide. The effects of the protein kinase C activator phorbol 12-myristate 13-acetate (10 μM) on H_2O_2-induced cell death were investigated. Phorbol 12-myristate 13-acetate sig-nificantly improved cell survival (49.2 % ± 8.1 % to 64.3 % ± 5.1 %, P < 0.01) while its inhibitor, chelerythrine (1μM), abrogated the effect. The phosphorylation of Bad at serine112 residue was augmented by phorbol 12-myristate 13-acetate; which was in- hibited by chelerythrine. Conclusions: Protein kinase C activator is useful to prevent cell death of tongue muscle-derived Sca-1(+) cells through the activation of Bad at ser- ine 112 residue.
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| 本文言語 | eng
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| 著者キーワード | stem cells
cell death
oxidative stress
protein kinase C
bad
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| 資料タイプ | text |
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| ファイル形式 | application/pdf |
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| 出版者 | Yamaguchi University School of Medicine
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| NII資料タイプ | 紀要論文 |
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| ISSN | 0513-1812
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| NCID | AA00594272
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| 学内刊行物(紀要等) | The bulletin of the Yamaguchi Medical School |
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| 掲載誌名 | The bulletin of the Yamaguchi Medical School |
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| 巻 | 60 |
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| 号 | 1-2 |
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| 開始ページ | 1 |
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| 終了ページ | 10 |
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| 発行日 | 2013 |
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| 著者版/出版社版 | 出版社版 |
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| リポジトリID | A050060000101 |
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| 地域区分 | 山口大学
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| URI | http://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A050060000101 |
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