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フルテキストURLA050060000101.pdf ( 1.4MB ) 公開日 2014-02-21
タイトルPhosphorylation of bad by protein Kinase-C activator can attenuate tongue muscle-derived stem cell death caused by oxidative stress
作成者Fukagawa, Yasuhiro
Miura, Toshiro
Shibuya, Masaki
Akashi, Shintaro
Oda, Takamasa
Nakamura, Takeshi
Matsuzaki, Masunori
Yano, Masafumi
作成者ヨミフカガワ, ヤスヒロ
ミウラ, トシロウ
シブヤ, マサキ
アカシ, シンタロウ
オダ, タカマサ
ナカムラ, タケシ
マツザキ, マスノリ
ヤノ, マサフミ
作成者別表記中村, 武史
矢野, 雅文
作成者所属山口大学医学部附属病院
山口大学大学院医学系研究科(医学)
内容記述(抄録等)Background: Improving the survival of transplanted cells is a critical is- sue in the use of stem cell transplantation therapy for cardiovascular disease. Many transplanted cells are lost through apoptosis or necrosis triggered by hypoxia or superoxide. Methods and Results: Tongue muscle-derived Sca-1(+) cells were isolated from the mouse and cultured for 2 weeks and then exposed to hydrogen peroxide (H_2O_2;0.2 mM) for 24 h. The extent of cell death by either apoptosis or necrosis was mea-sured by fluorescence-activated cell sorting using annexin V and propidium iodide. The effects of the protein kinase C activator phorbol 12-myristate 13-acetate (10 μM) on H_2O_2-induced cell death were investigated. Phorbol 12-myristate 13-acetate sig-nificantly improved cell survival (49.2 % ± 8.1 % to 64.3 % ± 5.1 %, P < 0.01) while its inhibitor, chelerythrine (1μM), abrogated the effect. The phosphorylation of Bad at serine112 residue was augmented by phorbol 12-myristate 13-acetate; which was in- hibited by chelerythrine. Conclusions: Protein kinase C activator is useful to prevent cell death of tongue muscle-derived Sca-1(+) cells through the activation of Bad at ser- ine 112 residue.
本文言語eng
著者キーワードstem cells
cell death
oxidative stress
protein kinase C
bad
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
60
1-2
開始ページ1
終了ページ10
発行日2013
著者版/出版社版出版社版
リポジトリIDA050060000101
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A050060000101