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フルテキストURLA020035000301.pdf ( 1.5MB ) 公開日 2010-04-19
タイトルExpression of MHC Antigents in Fetal Rat Thymus
作成者Kuniki, Hiromichi
Fujikura, Yoshihisa
Fukumoto, Tetsuo
作成者ヨミクニキ, ヒロミチ
フジクラ, ヨシヒサ
フクモト, テツオ
作成者所属山口大学医学部
内容記述(抄録等)Major histocompatibility complex (MHC) Class I antigen is known distinct on cell surface of the mature cells including lymphocytes but not detected on early stage of thymocytes. Monoclonal antibody, OX-18 or HAM-2 is the specific antibody to rat MHC Class I antigen and OX-6 is that to MHC Class II. These antibodies are detected immunohistochemically or flow cytofluorometrically by using HRP or FITC conjugated anti-mouse immunoglobulin. Main findings are as follows : MHC Class I antigen first appeared on thymic epithelial cells at day 15 of gestation and with the progress of fetal stage MHC Class I antigen positive cells appeared to increase in number and its intensity of the staining. MHC Class I antigen on thymocytes at about day 15 of gestation was negative, however at day 19 of gestation was about 36%, which is same as in normal young adult thymus. The degree of total antigen amount expressed in fetal thymus was compared with that in adult thymus, using radioactive binding inhibition assay. The amount of antigen is quite low at days 18 and 20 of gestation compared to that of adult. From these findings it is suspected that MHC Class I antigen first appeared on epithelial cells and a few days later on thymocytes. Thus, the interaction between MHC Class I positive epithelial cells and thymocytes may be important for the differentiation and maturation of thymocytes.
本文言語eng
著者キーワードHMC antigen
fetal thymus
Monoclonal antibodies
Thymic epithelial cells
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University Graduate School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
35
3-4
開始ページ35
終了ページ41
発行日1988-12
著者版/出版社版出版社版
リポジトリIDA020035000301
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020035000301