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フルテキストURLA020030000102.pdf ( 538.7KB ) 公開日 2010-04-19
タイトルA Sensitive Method for Detecting Sudunit Dissociation of Abnormal Hemoglabin
作成者Hattori, Yukio
Ohba, Yuzo
Miyaji, Takaoki
作成者ヨミハットリ, ユキオ
オオバ, ユウゾウ
ミヤジ, タカオキ
作成者所属山口大学医学部
内容記述(抄録等)Dissociation of abnormal hemoglobin (HbA) by gel permeation employing Sephadex G-75 column. Equimolar solutions(5чM)of Hb A and an abnolmal hemoglobin in 0.01 M phosphate buffer, 7.00 containing 0.09 M NaCl and a trace amount of KCM, are introduced into the column (1.0×60 cm,upward flow) by a peristatic pump (flow rate 0.25 ml/min) one after another. The absorbance at 280 nm or Soret band of the effluent is continuously monitored. The difference in the degree of dissociation is detected by the change in the absorbance at the interface where the two hemoglobins meet. The method developed here has been applied to fifteen different abnormal hemoglobins; twelve of them (80%) have shown either accelerated or reduced dissociation. The method is highly sensitive and specific for the detection of abnormal hemoglobins.
本文言語eng
著者キーワードAbnormal hemoglobin
Subunit dissociation
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
30
1
開始ページ11
終了ページ19
発行日1983-12
著者版/出版社版出版社版
リポジトリIDA020030000102
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020030000102