| フルテキストURL | A020023000302.pdf ( 4.2MB ) 公開日 2010-04-16
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| タイトル | Purification Method of Proteins by Preparative Gel Electrophoresis. Crystallisation of α-amylases. |
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| 作成者 | Takeo, Kazusuke
Suzuno, Ryosuke
Fujimoto, Masanori
Kuwahara, Akira
Ogata, Hatanori
Nakamura, Shojiro
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| 作成者ヨミ | タケオ, カズスケ
スズノ, リョウスケ
フジモト, マサノリ
クワハラ, アキラ
オガタ, ハタノリ
ナカムラ, ショウジロウ
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| 作成者所属 | 山口大学医学部
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| 内容記述（抄録等） | Taka-amylase A from Aspergillus orizae and huma α-amylase were crystallized in a three-step procedure: (1) Crude extract o fTaka-diastase (Sankyo) or saliva supernatant was applied to a gel slab type apparatus of preparative electrophoresis. The active fractions were extracted by crushing the gel slices with a buffer solution.(2) The soluble gel components, which were extracted from the gel slices together with protein fractions, were removed by electrophoresis with a discontinuous buffer system containing sucrose gradient (purification electrophoresis).(3) After acetone fractionation, crystalline amylases were obtained. The overall recovery rates of Taka-amylase A and human salivary amylase were 51% and 53%, respectively. Further usage of preparative gel electrophoresis and purification electrophoresis to obtaining pure proteins was examined.
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| 本文言語 | eng
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| 主題 | 医学
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| 資料タイプ | text |
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| ファイル形式 | application/pdf |
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| 出版者 | Yamaguchi University Graduate School of Medicine
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| NII資料タイプ | 紀要論文 |
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| ISSN | 0513-1812
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| NCID | AA00594272
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| 学内刊行物(紀要等) | The bulletin of the Yamaguchi Medical School |
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| 掲載誌名 | The bulletin of the Yamaguchi Medical School |
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| 掲載誌名別表記 | 山口医科大学紀要 |
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| 巻 | 23 |
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| 号 | 3-4 |
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| 開始ページ | 165 |
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| 終了ページ | 183 |
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| 発行日 | 1976-12 |
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| 著者版/出版社版 | 出版社版 |
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| リポジトリID | A020023000302 |
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| 地域区分 | 山口大学
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| URI | http://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020023000302 |
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