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タイトルComparison of bacterial community structures in the rhizoplane of tomato plants grown in soils suppressive and conducive towards bacterial wilt
作成者Shiomi, Yoshitaka
Nishiyama, Masaya
Onizuka, Tomoko
Marumoto, Takuya
作成者ヨミシオミ, ヨシタカ
ニシヤマ, マサヤ
オニズカ, トモコ
マルモト, タクヤ
作成者別表記丸本, 卓哉
作成者所属山口大学農学部
内容記述(抄録等)It has been reported that the growth of Ralstonia solanacearum is suppressed at the rhizoplane of tomato plants and that tomato bacterial wilt is suppressed in plants grown in a soil (Mutsumi) in Japan. To evaluate the biological factors contributing to the suppressiveness of the soil in three treated Mutsumi soils (chloroform fumigated soil; autoclaved soil mixed with intact Mutsumi soil; and autoclaved soil mixed with intact, wilt-conducive Yamadai soil) infested with R. solanacearum, we bioassayed soil samples for tomato bacterial wilt. Chloroform fumigation increased the extent of wilt disease. More of the tomato plant samples wilted when mixed with Yamadai soil than when mixed with Mutsumi soil. Consequently, the results indicate that the naturally existing population of microorganisms in Mutsumi soil was significantly able to reduce the severity of bacterial wilt of tomato plants. To characterize the types of bacteria present at the rhizoplane, we isolated rhizoplane bacteria and classified them into 22 groups by comparing their 16S restriction fragment length polymorphism patterns. In Yamadai soil a single group of bacteria was extremely predominant (73.1%), whereas in Mutsumi soil the distribution of the bacterial groups was much more even. The 16S rDNA sequence analysis of strains of dominant groups suggested that gram-negative bacteria close to the -proteobacteria were most common at the rhizoplane of the tomato plants. During in vitro assays, rhizoplane bacteria in Mutsumi soil grew more vigorously on pectin, one of the main root exudates of tomato, compared with those in Yamadai soil. Our results imply that it is difficult for the pathogen to dominate in a diversified rhizobacterial community that thrives on pectin.
本文言語eng
主題農学
資料タイプtext
出版者American Society for Microbiology
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN0099-2240
NCIDAA00543249
掲載誌名Applied and environmental microbiology
65
9
開始ページ3996
終了ページ4001
発行日1999-09
PubMed IDinfo:pmid/10473407
関連情報URL(IsPartOf)http://aem.asm.org/contents-by-date.0.shtml
著者版/出版社版その他
リポジトリID2007020081
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2007020081