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フルテキストURLA020010000304.pdf ( 1.1MB ) 公開日 2010-04-16
タイトルIn Vitro Cultivation of MN-Lymphosarcoma Cells
作成者Mori, Shigeki
Harada, Yoshio
Yamaoka, Ikuo
作成者ヨミモリ, シゲキ
ハラダ, ヨシオ
ヤマオカ, イクオ
作成者所属山口大学医学部
内容記述(抄録等)Fundamental conditions suitable for the cultivation of MN-lymphosarcoma cell were assayed and the results were presented as follow : 1) Static cultere was a more suitable method than roller tube cultere. 2) The cultere in initial pH 7.6 was found to be optimal. 3) Continuous cell multiplication was obtained with the inoculum size of over 100,000 nuclei/ml/tube. 4) 0.4% Lactalbumin hydrolysate in the medium was found to be optimal. 5) The optimal concentration of yeast extract was determined as 0.08% in the medium. 6) Vigorous propagation of the cells was obtained with a medium containing 40% bovine serum. 7) The cell multiplication was not sustained in the medium in whish dialysed bovine serum was substituted for nondialysed one. 8) The cell growth was accelerated markedly in the medium supplemented with dialysed bovine serum after the addition of hexestrol, a synthetic estrogen, to it.
本文言語eng
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University Graduate School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
10
3
開始ページ179
終了ページ191
発行日1963-09
著者版/出版社版出版社版
リポジトリIDA020010000304
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020010000304