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フルテキストURL2010010091.pdf ( 249.8KB ) 公開日 2010-06-25
タイトルXanthine oxidase is one of the major sources of superoxide anion radicals in blood after reperfusion in rats with forebrain ischemia/reperfusion
作成者Ono, Takeru
Tsuruta, Ryosuke
Fujita, Motoki
Shinagawa, Aki Hiromi
Kutsuna, Satoshi
Kawamura, Yoshikatsu
Wakatsuki, Jun
Aoki, Tetsuya
Kobayashi, Chihiro
Kasaoka, Shunji
Maruyama, Ikuro
Yuasa, Makoto
Maekawa, Tsuyoshi
作成者ヨミオノ, タケル
ツルタ, リョウスケ
フジタ, モトキ
シナガワ, アキ ヒロミ
クツナ, サトシ
カワムラ, ヨシカツ
ワカツキ, ジュン
アオキ, テツヤ
コバヤシ, チヒロ
カサオカ, シュンジ
マルヤマ, イクロウ
ユアサ, マコト
マエカワ, ツヨシ
作成者別表記小野, 建
鶴田, 良介
藤田, 基
忽那, 賢志
河村, 宜克
若月, 準
前川, 剛志
作成者所属山口大学医学部附属病院
山口大学大学院医学系研究科(医学)
内容記述(抄録等)We recently reported that excessive superoxide anion radical (O_2–•) was generated in the jugular vein during reperfusion in rats with forebrain ischemia-reperfusion using a novel electrochemical sensor and excessive O_2–• generation was associated with oxidative stress, early inflammation, and endothelial injury. However, the source of O_2–• was still unclear. Therefore, we used allopurinol, a potent inhibitor of xanthine oxidase (XO), to clarify the source of O_2–• generated in rats with forebrain ischemia-reperfusion. The increased O_2–• current and the quantified partial value of electricity (Q), which was calculated by the integration of the current, were significantly attenuated after reperfusion by pretreatment with allopurinol. Malondialdehyde (MDA) in the brain and plasma, High-mobility group box 1 (HMGB1) in plasma, and intercellular adhesion molecule-1 (ICAM-1) in the brain and plasma were significantly attenuated in rats pretreated with allopurinol with dose-dependency in comparison to those in control rats. There were significant correlations between total Q and MDA, HMGB, or ICAM-1 in the brain and plasma. Allopurinol pretreatment suppressed O_2–• generation in the brain-perfused blood in the jugular vein, and oxidative stress, early inflammation, and endothelial injury in the acute phase of forebrain ischemia-reperfusion. Thus, XO is one of the major sources of O_2–• in blood after reperfusion in rats with forebrain ischemia-reperfusion.
本文言語eng
著者キーワードallopurinol
electrochemical sensor
forebrain ischemia
reperfusion
superoxide anion radical
xanthine oxidase inhibitor
資料タイプtext
ファイル形式application/pdf
出版者Elsevier
NII資料タイプ学術雑誌論文
査読の有無査読あり
ISSN0006-8993
NCIDAA0057324X
掲載誌名Brain research : international multidisciplinary journal devoted to fundamental research in the brain sciences
1305
開始ページ158
終了ページ167
発行日2009-12-11
PubMed IDinfo:pmid/19781528
DOIinfo:doi/10.1016/j.brainres.2009.09.061
関連情報URL(IsPartOf)http://www.sciencedirect.com/science/journal/00068993
権利関係Copyright c2009 Elsevier Inc. All rights reserved.
著者版/出版社版著者版
リポジトリID2010010091
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/2010010091