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フルテキストURLA020031000101.pdf ( 1.0MB ) 公開日 2010-04-19
タイトルCell Cycle Dependent Con-A binding-Flow Cytometric Analysis-
作成者Sasaki, Kohsuke
Takahashi, Manabu
Ogino, Tetsuro
Murakami, Tomoyuki
Kawasaki, Shoji
Nagaoka, Sakae
作成者ヨミササキ, コウスケ
タカハシ, マナブ
オギノ, テツロウ
ムラカミ, トモユキ
カワサキ, ショウジ
ナガオカ, サカエ
作成者別表記佐々木, 功典
作成者所属山口大学医学部
内容記述(抄録等)Changes in lectin binding activity occuring the cell cycle were determined flow cytometrically in exponentaiiy growing YU-101 cells by a technique of gated analysis based on cellular DNA content after double staining of the cells with fluorescein isothiocyanate (FITC) and propidium iodide (PI). Fluorescence intensity from FITC conjugated concanavalin A (Con A) increased during the cell cycle and reached maximum in G2+M phase. Binding of the lectins was reduced by half in G1 phase after cell division. Binding activity of Con A and in YU-101 cells was linked closely with cell cycle. It is surrested that the nuclear changes are associated with alterations of the cellular membrane and that molecular communications between the cell membrane and the cell nucleus control periodic cell cycle. The methodology developed in these studies gives reliable data since a procedure for cell synchronization which brings out some effects on the normal function of the cell cycle is unnecessary.
本文言語eng
著者キーワードCon A
Flow cytometry
Cell Cycle
DNA
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University Graduate School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
31
1
開始ページ1
終了ページ5
発行日1984-12
著者版/出版社版出版社版
リポジトリIDA020031000101
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020031000101