| フルテキストURL | A020049000105.pdf ( 403.1KB ) 公開日 2010-04-19
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| タイトル | Establishment and Characterization of a New Human Pancreatic Cancer Cell Line, YPK-1 |
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| 作成者 | Yamamoto, Koutaro
Yahara, Noboru
Gondo, Toshikazu
Ishihara, Tokuhiro
Oka, Masaaki
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| 作成者ヨミ | ヤマモト, コウタロウ
ヤハラ, ノボル
ゴンドウ, トシカズ
イシハラ, トクヒロ
オカ, マサアキ
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| 作成者別表記 | 権藤, 俊一
岡, 正朗
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| 作成者所属 | 山口大学医学部
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| 内容記述（抄録等） | A new human pancreatic cancer cell line, designated YPK-1, was established from ascites collected from a 62-year-old Japanese man. This cell line has been maintained in vitro for 96 months with stable growth through 260 passages. We characterized the YPK-1 cell line morphology, doubling time, levels of tumor markers secreted into culture medium, chromosomal content, and tumorigenicity in nude mice. YPK-1 cells have a clear polygonal-shaped cytoplasm, round nuclei, and prominent nucleoli. The cells grew as an adherent monlayer and lacked contact inhibition. The doubling time at the tenth passage was 49.3 hours. YPK-1 cells secreted detectable amounts of carcinoembryonic antigen, carbohydrate antigen 19-9, carbohydrate antigen 125, carbohydrate antigen 50, DU-PAN-2, and Span-1. Chromosome analysis revealed that the diploid chromosome number ranged from 57 to 62 with a mode of 61. Tumorigenicity was demonstrated by development of tumors after subcutaneous injection of YPK-1 cells into nude mice. We believe that this cell line will contribute to studies of the biological and molecular properties of pancreatic carcinoma and the development of therapeutic strategies for this tumor.
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| 本文言語 | eng
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| 著者キーワード | Pancreatic cancer
Cell line
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| 主題 | 医学
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| 資料タイプ | text |
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| ファイル形式 | application/pdf |
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| 出版者 | Yamaguchi University Graduate School of Medicine
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| NII資料タイプ | 紀要論文 |
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| ISSN | 0513-1812
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| NCID | AA00594272
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| 学内刊行物(紀要等) | The bulletin of the Yamaguchi Medical School |
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| 掲載誌名 | The bulletin of the Yamaguchi Medical School |
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| 掲載誌名別表記 | 山口医科大学紀要 |
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| 巻 | 49 |
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| 号 | 1-2 |
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| 開始ページ | 33 |
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| 終了ページ | 42 |
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| 発行日 | 2002-06 |
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| 著者版/出版社版 | 出版社版 |
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| リポジトリID | A020049000105 |
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| 地域区分 | 山口大学
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| URI | http://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020049000105 |
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