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フルテキストURLA020049000105.pdf ( 403.1KB ) 公開日 2010-04-19
タイトルEstablishment and Characterization of a New Human Pancreatic Cancer Cell Line, YPK-1
作成者Yamamoto, Koutaro
Yahara, Noboru
Gondo, Toshikazu
Ishihara, Tokuhiro
Oka, Masaaki
作成者ヨミヤマモト, コウタロウ
ヤハラ, ノボル
ゴンドウ, トシカズ
イシハラ, トクヒロ
オカ, マサアキ
作成者別表記権藤, 俊一
岡, 正朗
作成者所属山口大学医学部
内容記述(抄録等)A new human pancreatic cancer cell line, designated YPK-1, was established from ascites collected from a 62-year-old Japanese man. This cell line has been maintained in vitro for 96 months with stable growth through 260 passages. We characterized the YPK-1 cell line morphology, doubling time, levels of tumor markers secreted into culture medium, chromosomal content, and tumorigenicity in nude mice. YPK-1 cells have a clear polygonal-shaped cytoplasm, round nuclei, and prominent nucleoli. The cells grew as an adherent monlayer and lacked contact inhibition. The doubling time at the tenth passage was 49.3 hours. YPK-1 cells secreted detectable amounts of carcinoembryonic antigen, carbohydrate antigen 19-9, carbohydrate antigen 125, carbohydrate antigen 50, DU-PAN-2, and Span-1. Chromosome analysis revealed that the diploid chromosome number ranged from 57 to 62 with a mode of 61. Tumorigenicity was demonstrated by development of tumors after subcutaneous injection of YPK-1 cells into nude mice. We believe that this cell line will contribute to studies of the biological and molecular properties of pancreatic carcinoma and the development of therapeutic strategies for this tumor.
本文言語eng
著者キーワードPancreatic cancer
Cell line
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University Graduate School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
49
1-2
開始ページ33
終了ページ42
発行日2002-06
著者版/出版社版出版社版
リポジトリIDA020049000105
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020049000105