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フルテキストURLA020013000204.pdf ( 369.3KB ) 公開日 2010-04-16
タイトルDetection of Leucine Aminopeptipase Isozyme by Agar-Gel Electrophoresis; with Reference to Various Diseases and Pregnancy
作成者Wajima, Takeshi
作成者ヨミワジマ, タケシ
作成者所属山口大学医学部
内容記述(抄録等)A method for detecting leucine aminopeptidase (LAP) isozyme in human serum using agar gel electrophoresis was developed. This method excels other methods heretofore in use in simplicity and efficiency. By this method LAP isozyme can be detected in normal human serum in the α_1-globulin fraction or in the position intermediate between albumin and α_1-globulin of serum protein. LAP activity bands can be detected in positions corresponding to albumin and postalbumin of serum protein with liver homogenate, in positions corresponding to α_2-globulin and β-globulin fractions with pancreatic homogenate, in a position corresponding to α_1 globulin fraction with duodenal and renal homogenates, and positions corresponding to α_1-globulin and α_2 globulin fractions with bile homogenate. The serum leucine aminopepidase zymograms of 200 cases of various diseases, including 123 cases of hepatobiliary diseases, and 48 pregnant women were observed. LAP zymograms of normal human serum are of two types, namely Aα_1 type and α_1 type, but in hepatobiliary diseases 1 or 2 other LAP activity bands appear so that zymograms are different, I.e., Aα_1+α_1 type or Aα_1+α_2+α_2+β type. LAP zymograns were mainly Aα_1 type in such conditions as hepatitis and liver cancer which are for the most part distubances of the liver parenchyma, but almost all hepatobiliary obstruction diseases due to cholelithiasis or cancer presented the Aα_1+α_2 and Aα_1+α_2+α_2+β types. The Aα_1+α_2+α_2+β type is peculiar to bile duct obstruction. The serum of women in the latter half of pregnancy (after the sixth month) showed just one activity band (α_2 type) in a position corresponding to the position of α_2 globulin in the serum.
本文言語eng
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University Graduate School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
13
2
開始ページ83
終了ページ88
発行日1966-06
著者版/出版社版出版社版
リポジトリIDA020013000204
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020013000204