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フルテキストURLA020038000106.pdf ( 2.1MB ) 公開日 2010-04-19
タイトルKaryological Variation of Clonal Malignant Cell Lines
作成者Sasaki, Kazuhiko
Nakada, Taishi
Tanaka, Rikuo
Honjou, Kazumitsu
Homma, Yutaka
Nakanishi, Takashi
Murakami, Tomoyuki
作成者ヨミササキ, カズヒコ
ナカダ, タイシ
タナカ, リキオ
ホンジョウ, カズミツ
ホンマ, ユタカ
ナカニシ, タカシ
ムラカミ, トモユキ
作成者所属山口大学医学部
内容記述(抄録等)Chromosomal analysis and DNA flow cytometry were performed on HeLa, malignant fibrous histiocytoma (MFH) and their clonal sublines. Two HeLa clones attained confluency in a 60-mm dish on the 45th day after cloning. In MFH two clonal sublines began to increase cell numbers at the first. One continued growth and became confluent on the 177th day but the other ceased to grow. The chromosome number in all parent and clonal sublines showed a wide range of distribution, mostly in the hypotriploid range. In spite of single cell origen, remarkable karyological variation were seen in all clonal sublines. DNA histogram of all tumor lines measured by flow cytometry displayed distinct G1-peak which was narrower than expected from the distribution of chromosome numbers. All G1-peak as well as modal chromosome numbers were in hyportriploid region. From karyological variation in clonal sublines, chromosomal alteration may occur during growth. Furthermore, it is suggestive that the cell with near-triploid might be advantageous for proliferation, so that the cell with extremely small and large number of chromosomes can not progress.
本文言語eng
著者キーワードClone
Malignant cell
Karyotype
Instability
Flow cytometry (FCM)
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University Graduate School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
38
1-2
開始ページ33
終了ページ40
発行日1991-06
著者版/出版社版出版社版
リポジトリIDA020038000106
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020038000106