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フルテキストURLA020015000306.pdf ( 598.4KB ) 公開日 2010-04-16
タイトルDirect Determination of Blood Ammonia by Berthelot’s Coloraiton of Protein-free Supernatant Obtained by Folin-Wu Deproteinizaion
作成者Kitajima, Kazuhiko
Miyamura, Sigenori
Murakawa, Setsuko
作成者ヨミキタジマ, カズヒコ
ミヤムラ, シゲノリ
ムラカワ, セツコ
作成者所属山口大学医学部
内容記述(抄録等) A direct determination of blood ammonia circumventing the use of microdiffusion apparatus which was devised by Fujii consists in (1) deproteinization of blood by a modified Folin-Wu's procedure, (2) coloration of thus deproteinized supernatant of blood by the Berthelot reaction and (3) colorimetry. This is a simple and convenient method, but our experience disclosed several drawbacks in it. A series of experiments were carried out in our laboratory in the hope that Fujii's method might be improved. The following is the outline of our improvement. 1゜. Collection of blood sample. Blood (about 3 ml) is withdrawn with a syringe by venous puncture, transferred into a test tube containing the crystals of potassium oxalate, stirred gently to prevent coagulation, and immediately cooled by inserting into a pile of ice cubes so that release of ammonia from non-NH_3 nitrogen substances may be stopped completely. 2゜. Deproteinization. To the blood (1.0 ml) thus collected are added 10 g/dl sodium tungstate solution (0.5 ml) and 1 N sulfuric acid (0.5 ml), allowed to stand (for 15 minutes), and centrifuged to get clear supernatant. (This procedure of deproteinization insures the elimination of danger for the spontaneous increase in ammonia reading which may arise while the mixture of blood with deproteinzing reagent is allowed to stand at room temperature). 3゜. Coloration. The deproteinzed supernatant of blood (0.5 ml) is mixed with phenol-nitroprusside solution (2.0 ml) and antiformin solution (2.0 ml), mixed and incubated at 37℃ (for 3 minutes). (A stable blue coloration which is not disturbed by the co-existing non-NH_3 nitrogen substances is obtained in this way.) 4゜. The standard solutions of ammonium sulfate (1.0 ml aliquots ; 100, 400mg N/dl) are treated with deproteinizing reagents and color reagents in the same way as described above (3゜) in order to construct a calibration curve. 5゜. Colorimetry at 630 mμ.
本文言語eng
主題医学
資料タイプtext
ファイル形式application/pdf
出版者Yamaguchi University Graduate School of Medicine
NII資料タイプ紀要論文
ISSN0513-1812
NCIDAA00594272
学内刊行物(紀要等)The bulletin of the Yamaguchi Medical School
掲載誌名The bulletin of the Yamaguchi Medical School
掲載誌名別表記山口医科大学紀要
15
3
開始ページ207
終了ページ217
発行日1968-09
著者版/出版社版出版社版
リポジトリIDA020015000306
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/A020015000306