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タイトル土壌のATP含量による微生物バイオマスの測定
タイトルヨミドジョウ ノ ATP ガンリョウ ニヨル ビセイブツ バイオマス ノ ソクテイ
タイトル別表記Estomation of microbial biomass from ATP content in soil
作成者丸本, 卓哉
伊藤, 文
作成者ヨミマルモト, タクヤ
イトウ, アヤ
作成者別表記Marumoto, Takuya
Itoh, Aya
作成者所属山口大学農学部
内容記述(抄録等)A newly developed extractant of soil-ATP (TEA/NRB reagent) was compared with an usual extractant of TCA/paraquat on their extractability of microbial ATP in soils and some experimental conditions in the soil-ATP easurement by the TEA-NRB method was established. The TEA/NRB selectively extracted the ATP from microbial cells, although the TCA/paraquat extracted the ATP not only from microbial cells but also from root heirs and nematodes in soils. Some experimental conditions in the TEA method, i. e. the rate of the TEA/NRB reagents to soils, time of ultrasonic treatment and temperature were examined and a rebised procedure of the ATP measurement in soil was proposed. A better correlation between the soil-ATP content by TEA method and the amount of biomass C by chloroform fumigation method was obtained. A mean factor for calculation from ATP content to biomass C was 870 for soils without cropping and c.a. 1100 under cropping. The calculation factor may be depended on the supply of energy source to microbes, cultivation, organic matter content and clay minerals in soil. These results suggest that the TEA method is very useful to measure soil microbial ATP and to estimate microbial biomass and it can be applied to the root-zone soil.
本文言語jpn
主題農学
資料タイプtext
出版者山口大学農学部
出版者ヨミヤマグチ ダイガク ノウガクブ
NII資料タイプ紀要論文
ISSN0513-1715
NCIDAN00244250
学内刊行物(紀要等)山口大学農学部学術報告
掲載誌名山口大学農学部学術報告
掲載誌名別表記Bulletin of the Faculty of Agriculture, Yamaguchi University
35
開始ページ87
終了ページ106
発行日1987
著者版/出版社版その他
リポジトリIDD490035000007
地域区分山口大学
URIhttp://www.lib.yamaguchi-u.ac.jp/yunoca/handle/D490035000007